Anti-human immunodeficiency virus-1 activities of pratensein and pratensein glycoside from Alhaji maurorum and its parasite Cuscuta kotchiana

Abstract

Objective: To test the anti-human immunodeficiency virus (HIV) activity of pure compounds isolated from aerial part extracts of Alhaji maurorum and its parasite Cuscuta kotchiana. Methods: The anti-HIV-1 and anti-HIV-2 activities of these extracts were performed by use of quantitative polymerase chain reaction assay and high pure viral nucleic acid kit. The most active fractions against HIV-1 were detected by nuclear magnetic resonance as pratensein and pratensein glycoside respectively in A. maurorum and C. campestris. Results: These two extracts have low toxicity on HIV-2 replication. The 50% effective concentration for HIV-1 replication of pratensein and pratensein glycoside were 100 and 22 μg/mL, respectively. The time of addition assay showed that pratensein and pratensein glycoside were most effective when added at the early stage (0–4 h) of virus replication. Conclusion: The pratensein glycoside inhibits HIV-1 replication in host cells more than pratensein and both extracts are potent inhibitors of HIV-1 entry. © 2017 Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag GmbH Germany