Cloning and expression of vegflllb, A new isoforms of VEGF family with antiangiogenic activity

Abstract

Vascular endothelial growth factors (VEGF-A) are a family of growth factors that stimulate angiogenesis in tumor growth and progression. The VEGF-A isoforms have pro-angiogenic effect. Another family of VEGF-A isoforms termed VEGFxxxb isoforms which contain antiangiogenic effects. Here we designed and expressed a new member of VEGFxxxb family, termed VEGFlllb. VEGFlllb was designed and cloned in pBUDce4.l expression vector to transfect HEK293 cells. Transiently transfected cells were examined by both real time PCR and western blot, to assess the VEGFlllb expression. Data were eveident that the recombinant VEGFlllb was successfully expressed in HEK293 cells. There was a significant difference in expression of VEGF gene in the transfected group than in the control group (l.3±0.l, P=0.03). Collectively there is no VEGFlllb primial expression in HEK293 cells and these cells are able to produce the recombinant VEFGlllb which is accounted for trapeutic applications. © OmicsVista Group, All rights reserved.