Design and construction of a label free aptasensor for electrochemical detection of sodium diclofenac

Abstract

The present manuscript describes a label free electrochemical aptasensor for the detection of sodium diclofenac (DCF). In order to construct the biosensor, the amino-functionalized diclofenac binding aptamer (DBA) was covalently immobilized on the surface of the glassy carbon electrode (GCE). The conformation of the DBAs on the surface of the electrode is changed when this is exposed to different concentrations of DCF. The introduction of DCF induces an alteration in the conformation of the surface immobilized DBA and causes a decrease in the charge transfer resistance of the aptasensor. However, the charge transfer resistance is increased by incubation of GCE/DBA/DCF in the secondary DBA. The changes in the charge transfer resistance have been monitored using the voltammetric and electrochemical impedance spectroscopic (EIS) techniques. The aptasensor shows two different linear dynamic ranges over 0-5.0μM and 10μM to 1mM, and the sensitivity of 15.7kΩμM -1 and detection limit of 2.7×10 -7M were obtained. The validity of the method and applicability of the aptasensor were successfully evaluated by detection of DCF in a blood serum sample without interference from the sample matrix. Furthermore, the aptasensor has shown good stability. © 2012 Elsevier B.V.