FcUni-RLuc: An engineered Renilla luciferase with Fc binding ability and light emission activity

Abstract

A novel and advanced Fc-binding probe-FcUni-RLuc namely-has been produced and functionally assayed for labelling IgGs. The Fc antibody binding sequence-HWRGWV-was fused to Renilla luciferase, and the purified probe was employed for bioluminescence enzyme-linked immunoabsorbance assay of Her2 positive cells. This journal is © The Royal Society of Chemistry.