Heteroalkyl oxime derivative can cause damage to bacterial DNA

Abstract

The present study aimed to evaluate the antimicrobial activity of a hetroalkyl oximec synthetic compound derivative (Benzophenone O-4-bromoethyl oxime) against two gram positive and three gram negative bacteria. The antibacterial characteristic of this compound was shown using the circular zone diameter of bacterial growth inhibition by disk-diffusion method. In addition, the random amplified polymorphic DNA (RAPD) technique using twenty-three primers was performed to investigate the DNA polymorphism of bacteria that were affected by the synthetic chemical drug. Among these, only 18 primers were found efficient for reproducible banding pattern. Disappearance or appearance of bands between controls and treatments confirm rearrangements and DNA damages in the priming binding sites of bacterial genome. The results show that the RAPD method can be a useful identification tool for studying the DNA polymorphism created by synthetic chemical drugs. The results obtained with this technique showed significant differences between the RAPD profiles.