Type: Article

In vitro cytotoxicity studies of palladacyclic complexes containing the symmetric diphosphine bridging ligand. Studies of their interactions with DNA and BSA

Journal: European Journal of Medicinal Chemistry (02235234)Year: 12 February 2014Volume: Issue: Pages: 8 - 17

Karami, KazemKarami, KazemHosseini-Kharat, MahboubehHosseini-Kharat, MahboubehSadeghi H.^aSadeghi H.^a Mirian M. Mirian M.

a :University of Isfahan - IRAN(IR) - Isfahan

DOI:10.1016/j.ejmech.2013.11.042Language: English

Abstract

The reactions between [Pd2{(C,N)-C6H 4CH2NH(Et)}2(μ-X)2] (X = Cl or Br) and 1,2-bis(diphenylphosphino)ethane (dppe) in the 1:1 molar ratio resulted in the dppe-bridged Pd(II) complexes, [Pd2{(C,N)-C6H 4CH2NH(Et)}2(μ-dppe)(Cl)2] (1) and [Pd2{(C,N)-C6H4CH2NH(Et)} 2(μ-dppe)(Br)2] (2), respectively, which were characterized by elemental analyses, infrared (IR), 1H- and 31P{1H} NMR spectroscopy. The molecular structure of 1 was determined by single-crystal X-ray diffraction. In vitro cytotoxicity of 1, 2, dppe, PhCH2NH(Et) and cisplatin were carried out against four human tumor cell lines. The interactions of complexes towards DNA and protein are investigated. The results suggested that both complexes could interact with FS-DNA through the intercalation mode. Moreover, the reactivity towards BSA revealed that the microenvironment and the secondary structure of BSA were changed in the presence of Pd(II) complexes.© 2013 Elsevier Masson SAS. All rights reserved.