Detection of aflD gene in contaminated pistachio with Aspergillus flavus by DNA based electrochemical biosensor

Abstract

Aspergillus flavus detection and the removal of contaminated pistachio are critically important. In this work, an impedimetric electrochemical DNA sensor using gold nanoparticles on the glassy carbon electrode was developed for the sensitive detection of the aflD gene of A. flavus. This biosensor had a linear range of the detection, which was from 1 nM to 10 µM with the detection limit of 0.55 nM, while the best time for hybridization was 4 hr. Results for the detection and reproducibility of the synthetic 21-nucleotide target and the target 76-nucleotide polymerase chain reaction product were the same due to the sensitivity of measurement. In addition, this biosensor can be used to enumerate A. flavus up to 2.5 × 108 spores/mL. © 2017 Taylor & Francis Group, LLC.