Direct Voltammetry of Copper, Zinc-Superoxide Dismutase Immobilized onto Electrodeposited Nickel Oxide Nanoparticles: Fabrication of Amperometric Superoxide Biosensor

Abstract

Direct electron transfer of immobilized copper, zinc-superoxide dismutase (SOD) onto electrodeposited nickel-oxide (NiOx) nanoparticle modified glassy carbon (GC) electrode displays a well defined redox process with formal potential of -0.03V in pH7.4. Cyclic voltammetry was used for deposition of (NiOx) nanoparticles and immobilization of SOD onto GC electrode. The surface coverage (Γ) and heterogeneous electron transfer rate constant (ks) of immobilized SOD are 1.75×10-11molcm-2 and 7.5±0.5s-1, respectively. The biosensor shows a fast amperometric response (3s) toward superoxide at a wide concentration range from 10μM to 0.25mM with sensitivity of 13.40nAμM-1cm-2 and 12.40nAμM-1 cm-2, detection limit of 2.66 and 3.1μM based on anodically and cathodically detection. This biosensor exhibits excellent stability, reproducibility and long life time. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.