Effect of bovine amniotic fluid on intra-abdominal adhesion in male rats

Abstract

Background: A wide variety of treatments have been proposed in order to deal with prevention of postoperative adhesions formation, but, no definitive results have been achieved. In the present study, bovine amniotic fluid (BAF) has been investigated as a possible option. Materials and methods: 84 male Wistar rats were undergone a laparotomy. After 2 weeks, adhesions were scaled grossly. Bovine amniotic fluid (in whole combination, without cells or without cells and proteins) extracted from cows carried either male or female calves was then applied to treated groups 2 weeks later during the second laparotomy. Adhesions were rescored 2 weeks later during a third laparotomy. Results and conclusion: A significant reduction (P< 0.05) in adhesions formation was observed only in rats treated with male bovine amniotic fluid without cells and proteins. Therefore, BAF could be used in treatment of adhesion formation because it is inexpensive, readily available, and has minimal side effects. © 2010 Surgical Associates Ltd.