Endogenous expression of the atypical chemokine receptor CCX-CKR (CCRL1) gene in human embryonic kidney (HEK 293) cells

Abstract

CCX-CKR (CCRL1) as one of the chemokine receptor-like proteins is a scavenger of CCL19, CCL21, CCL25, and CXCL13 chemokines. Human CCX-CKR is expressed in various tissues. Since HEK 293 cells are used for both transient and stable expression of CCX-CKR gene, it is important to determine endogenous expression of CCX-CKR gene. Therefore, in the current study endogenous expression of CCX-CKR gene was evaluated in HEK 293 cells. To test the expression of CCX-CKR gene in HEK 293 cells, total RNA was isolated from HEK 293 cells and RT-PCR reaction was primed with the gene-specific primers. Protein expression is then evaluated by Western blot analysis and flow cytometry. Results of this study show that HEK 293 cells express an endogenous CCRL1 gene only at mRNA level. These data therefore represent the important implications for the use of HEK 293 cells as a host cell system for the study of CCX-CKR. © 2015, Springer Science+Business Media New York.