Immobilization of flavine adenine dinucleotide onto nickel oxide nanostructures modified glassy carbon electrode: Fabrication of highly sensitive persulfate sensor

Abstract

A simple method was used to fabricate flavin adenine dinucleotide (FAD)/NiOx nanocomposite on the surface of glassy carbon (GC) electrode. Cyclic voltammetry technique was applied for deposition nickel oxide nanostructures onto GC surface. Owing to its high biocompatibility and large surface area of nickel oxide nanomaterials with immersing the GC/NiOx-modified electrode into FAD solution for a short period of time, 10-140 s, a stable thin layer of the FAD molecules immobilized onto electrode surface. The FAD/NiOx films exhibited a pair of well-defined, stable, and nearly reversible CV peaks at wide pH range (2-10). The formal potential of adsorbed FAD onto nickel oxide nanoparticles film, E o′ vs. Ag/AgCl reference electrode is -0.44 V in pH 7 buffer solutions was similar to dissolved FAD and changed linearly with a slope of 58.6 mV/pH in the pH range 2-10. The surface coverage and heterogeneous electron transfer rate constant (k s) of FAD immobilized on NiOx film glassy carbon electrode are 4.66×10 -11 mol cm -2 and 63± 0.1 s -1, indicating the high loading ability of the nickel oxide nanoparticles and great facilitation of the electron transfer between FAD and nickel oxide nanoparticles. FAD/NiOx nanocomposite-modified GC electrode shows excellent electrocatalytic activity toward S 2O 8 2- reduction at reduced overpotential. Furthermore, rotated modified electrode illustrates good analytical performance for amperometric detection of S 2O 8 2-. Under optimized condition, the concentration calibration range, detection limit, and sensitivity were 3 μM- 1.5 mM, 0.38 μM and 16.6 nA/μM, respectively. © Springer-Verlag 2010.