Novel chitosan-Nafion composite for fabrication of highly sensitive impedimetric and colorimetric As(III) aptasensor

Abstract

In the present work, for the first time we takes the advantages of chitosan-Nafion (Chit-Naf) composite as a highly conductive surface platform and a novel CNT-based signal amplification strategy to develop a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III). The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays. In this work, we employed a signal amplification strategy based on carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system, by which sensitivity and detection limit of the aptasensor for the detection of As(III) were obtained to be 100.82 Ω nM −1 and a of 74 pM, respectively. This protocol provided one of the lowest limits of detection for As(III) on aptamer-based electrodes recently described in the literature. Moreover, the change of the optical absorptive properties of CNTs upon biorecognition interactions provides a way to detect the biorecognition process and thus allowed us to design an optical As(III) aptasensor using the UV–Vis spectroscopic method. The discrimination capability of the fabricated aptasensor for recognizing As(III) in the presence of other metal ions and a complex matrix of waste water samples was successfully investigated. This protocol provided a new method for sensitive detection of As(III) with considerable advantages in terms of reproducibility, selectivity, being mediator free and regenerability of the sensing interface. © 2019 Elsevier B.V.