Background
Type: Article

Separation and determination of sugars by reversed-phase high-performance liquid chromatography after pre-column microwave-assisted derivatization

Journal: Analytical and Bioanalytical Chemistry (16182642)Year: 2006/02/01Volume: Issue: 3
Momenbek F.a Khorasani J.H.
DOI:10.1007/s00216-005-0207-2Language: English

Abstract

In this study the possibility of derivatizing sugars using microwave irradiation was investigated. The amount of reagent, irradiation intensity, and derivatization time were optimized. In the derivatization of sugars with p-nitroaniline the reaction is complete within 5min at 600W when the p-nitroaniline-to-sugar and NaBH3CN-to-sugar mole ratios were above 1.4 and 3.1, respectively. A Doehlert design was used to optimize the mobile phase for separation of p-nitroaniline-labeled sugars; and the best separation was obtained by use of 0.01molL-1 acetate buffer at pH 4.40 containing 11.0% acetonitrile. Analysis using this method was highly sensitive and analysis time was short. Finally, a food sample was analyzed using the proposed method.