Background
Type: Article

Alpha-amylase immobilized on polycaprolactone-grafted magnetic nanoparticles: improving stability and reusability

Journal: Journal of Chemical Technology and Biotechnology (02682575)Year: 1 August 2020Volume: 95Issue: Pages: 2243 - 2250
Defaei M.Taheri Kafrani A.aMiroliaei M.a Yaghmaei P.
DOI:10.1002/jctb.6412Language: English

Abstract

BACKGROUND: Widespread application of enzymes for research and industrial purposes is dependent on enhancing the catalytic capacity. One promising approach to this end is the immobilization of enzymes on novel nanomaterials. In this study, a novel nanocarrier was developed for the immobilization of enzyme on polycaprolactone (PCL)-functionalized magnetic nanoparticles. RESULT: Structural and physicochemical characterization of the PCL-modified magnetic nanocarrier was achieved via Fourier-transform infrared (FTIR) spectra, scanning electron microscopy (SEM), transmission electron microscopy (TEM), thermogravimetric analysis (TGA) and vibration sample magnetometry (VSM). In order to determine the optimal conditions for immobilization, different parameters such as pH, temperature, reaction time and enzyme concentration were evaluated. Based on the results, the optimal biocatalytic activity of immobilized α-amylase was obtained at 65 °C and pH 6.5. Under appropriate conditions, the immobilized α-amylase showed improved storage stability in comparison with free one. Additionally, the reusability of the immobilized system demonstrated >50% enzyme activity, at optimal pH and temperature, after five reaction cycles. CONCLUSION: This finding could be useful in the application of immobilized enzymes for analytical purposes, industrial exploitations, biotechnology, biomedical engineering and other bio-applications. © 2020 Society of Chemical Industry. © 2020 Society of Chemical Industry


Author Keywords

catalytic activitymagnetic nanoparticlespolycaprolactonereusabilityα-amylase

Other Keywords

AmylasesBiomedical engineeringCatalyst activityChemical industryEnzyme activityEnzyme immobilizationFourier transform infrared spectroscopyHigh resolution transmission electron microscopyIndustrial researchMagnetismPolycaprolactoneReusabilityScanning electron microscopyThermogravimetric analysisVibration analysisamylaseimmobilized enzymemagnetic nanoparticlenanocarrierBiocatalytic activityEnzyme concentrationsFourier transform infraredMagnetic nanocarrierOptimal conditionsPhysico-chemical characterizationVibration sample magnetometryArticlebiocatalystcomparative studypHphysical chemistryreaction timetemperaturethermogravimetrythermostabilitytransmission electron microscopyvibrating sample magnetometryMagnetic nanoparticles