Characterization of three species of Dunaliella salina, Dunaliella parva and Dunaliella psuedosalina isolated from salt marsh of Gave khoni of Isfahan-Iran

Abstract

The effect of pH (ranging from 3 to 10), salinity (0.5, 2 and 4M NaCl) and light intensity (20, 60 and 300μmol quanta. m-2. s-1) on the growth curve of three species of D. salina, D.parva and D.psuedosalina were studied. Also, the effect of high temperature ranging from 35 to 60 °C on the motility and integrity of these three species at different salinity of 0.1, 0.5, 1, 1.5, 2, 3 and 4M NaCl in the medium, were studied. The results show that D.salina and D.parva could grow at pH ranges of 6 to 9 with optimum of 7 to 8 and D.psuedosalina at pH of 5 to 10 with optimum of 7 to 8. All three species had optimum growth rate at about 0.5 to 2.0M NaCl. The results also show that, in all three species cell motility and resistance to high temperature increased with increasing NaCl concentration in the medium.