Genetic variation of informative short tandem repeat (STR) loci in an Iranian population

Abstract

In the present study, genotyping of six short tandem repeat (STR) loci including CSF1PO, D16S539, F13A01, F13B, LPL and HPRTB was performed on genomic DNA from 127 unrelated individuals from the Iranian province of Isfahan. The results indicated that the allele and genotype distributions were in accordance with Hardy-Weinberg expectations. The observed heterozygosity (Ho), expected heterozygosity (He) as well as forensic and paternity indices including power of discrimination (PD) and exclusion (PE), polymorphism information content (PIC), typical paternity index (PItypical) and probability of paternity (W) were determined for the examined STR alleles. In addition, genetic diversity index (GD) and population parameter (θ) were calculated for the six loci. The combined power of discrimination (Pd combined) and combined probability of exclusion (PE typical) were 0.9999998 and 0.999856 over the six loci, respectively. Together, the examined STR loci in this study have proven a relatively high genetic variation in Iranian population. The data could be used for construction of a forensic genetic database for Iranian population.