Secondary Metabolites and Antioxidant Activity in Different Iranian Accessions of Dorema ammoniacum D. Don and Dorema aucheri Boiss.

Abstract

This study was performed to evaluate different secondary metabolites (SMs) and antioxidant activity in 14 different genotypes from methanolic extracts of plant samples (leaves and stem) from Dorema ammoniacum D. Don (D.am) and D. aucheri Boiss. (D.au). The contents of SMs and antioxidant activity were measured using UV-visible spectrophotometer. The highest total phenolics content (34.3 mg/GAE DW) and antioxidant activity (92%) through DPPH method were detected in leaves samples of D.au9 genotype. Highest levels of total flavonoids (5.5 mg QE/g DW) and total flavonols (3.6 mg QE/g DW) were observed in leaves of D.au12 and D.au2, respectively. The highest content of anthocyanin (250 nano mol/g FW) and carotenoids (18 mg/g DW) were found in leaves samples of D.au7 and D.au3 genotypes. The results showed the superiority of D. aucheri for different SMs rather than D. ammoniacum Finally, leaves samples of D. aucheri and D. ammoniacum species showed higher contents for secondary metabolites which may be suggested for further appliances with medicinal and industrial aims. © 2012 Iranian Society of Medicinal Plants. All rights reserved.