New Robust Redox and Conducting Polymer Modified Electrodes for Ascorbate Sensing and Glucose Biosensing

Abstract

A novel modified electrode with a conducting film containing poly(3,4-ethylenedioxythiophene) (PEDOT) plus poly(methylene blue) (PMB) on a glassy carbon electrode (GCE) (PEDOT/PMB/GCE) has been developed, and application illustrated as electrochemical sensor for ascorbate and biosensor for glucose. Electrocatalytic oxidation at 0.0V vs. SCE was successfully used for the determination of ascorbate in real samples. Glucose biosensors containing glucose oxidase (GOx) immobilized on PMB/PEDOT electrodes exhibit enhanced sensitivity relative to PEDOT ones. The new robust biosensor architecture shows a far superior operational and storage stability relative to PMB alone, enabling excellent preservation of enzyme activity during more than one month. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.