Research Output
Articles
Publication Date: 2025
Clinical and Experimental Dental Research (20574347)11(3)
Objectives: The selection of appropriate biomaterial for guided bone regeneration is challenging. The blending of polymers is a simple method to retain their characteristics and to compensate for the drawbacks of each component. The release of Sr+2 (strontium) ions from the polycaprolactone/strontium carbonate (PCL/SrC) nanocomposite is the main reason of enhanced osteogenesis. The most important reasons of clinical failure after using biomaterials include infections and lack of tissue-integration. Modifications of silk fibroin (SF)–based membranes improved new bone formation in animal studies without inflammatory reaction. The aim of the present study was to compare biological response of the subcutaneous connective tissue to a novel bilayer PCL (60 wt%)/SF (20 wt%)/SrC (20 wt%) membrane to a commercially available collagenous membrane. Material and Methods: Eighteen male New Zealand rabbits were randomly divided into three groups, and all received subcutaneously the following materials: novel bilayer membrane, commercial membrane, and empty defect as control group, which were tested after 7, 14, and 28 days. The type and severity of inflammation, granulation tissue, and fibrous tissue were assessed. Results: The connective tissue surrounding the implanted samples of each group exhibited the presence of similar cells close to the control groups. Statistical analyses showed no significant differences between the specimens in each time period. Conclusions: In general, the novel bilayer nanocomposite membrane was a biocompatible material and produces a similar subcutaneous response compared to commercially available membrane. Besides, it demonstrated promise for guided bone regeneration technique for treating the osseous defects of oral and maxillofacial region. © 2025 The Author(s). Clinical and Experimental Dental Research published by John Wiley & Sons Ltd.
Mehdikhani, M.,
Yilgör, P.,
Poursamar, S.A.,
Etemadi, N.,
Gokyer, S.,
Navid, S.,
Farzan, M.,
Farzan, M.,
Babaei, M.,
Rafienia m., M. Publication Date: 2024
International Journal of Biological Macromolecules (01418130)282
Skin injuries resulting from physical trauma pose significant health risks, necessitating advanced wound care solutions. This investigation introduces an innovative bilayer wound dressing composed of 3D-printed propolis-coated polycaprolactone (PCL/PP) and an electrospun composite of polyvinyl alcohol, chitosan, polycaprolactone, and diltiazem (PVA/CTS/PCL/DTZ). SEM analysis revealed a bilayer structure with 89.23 ± 51.47 % porosity and uniformly distributed nanofibers. The scaffold tensile strength, with pore sizes of 100, 300, and 500 μm, was comparable to native skin. However, smaller pore sizes reduced water vapor transmission from 4211.59 ± 168.53 to 2358.49 ± 203.63 g/m2. The incorporation of DTZ lowered the contact angle to 35.23 ± 3.65°, while the addition of PCL reduced the degradation rate and modulated the release of DTZ by approximately 50 %. Moreover, lower pH increased the degradation rate and decreased swelling. The inclusion of propolis enhanced antibacterial activity, and 10 % DTZ promoted the viability, proliferation, and migration of fibroblasts and adipose-derived stem cells. However, increasing DTZ concentration to 12 % reduced cell viability. In vivo tests on rats demonstrated effective wound healing and anti-inflammatory properties of the bilayer samples. Regarding the aforementioned results, the PCL/PP-PVA/CTS/PCL/DTZ (10 % w/w) bilayer wound dressing is a promising candidate for wound healing applications. © 2024
Heidarian loloei, Y.,
Mehdikhani, M.,
Askari, V.R.,
Zargar kharazi, A. Publication Date: 2024
Materials Today Chemistry (24685194)36
Skin treatment complexity and expense in accidents such as burns, lacerations, and illnesses represent a significant concern. The demand for nanofibers possessing a high biocompatible matrix and the ability to carry drugs to improve wound healing is highly regarded. In this investigation, electrospun nanofibers were fabricated utilizing a 17 % (w/v) polycaprolactone (PCL) core and a 2 % (w/v) chitosan (CS) shell, encompassing varying concentrations of curcumin (CUR, 10 μM, 25 μM, and 50 μM), and their structural, physicochemical, mechanical, and in vitro characteristics of the prepared samples were thoroughly examined. A TEM image indicated the mean thickness of the core and shell in the drug-loaded sample to be approximately 60 nm and 170 nm, respectively. Smooth nanofibers with a bead-free shape structure were also discernible through SEM evaluation, with a porosity of 84.27 ± 5.32 % for the PCL/CS/CUR25μM sample. Moreover, the addition of CUR exhibited tensile properties within the range of the skin's mechanical characteristics, along with suitable wettability (40.80 ± 2.99°), water absorption (858.82 ± 79.06 %), and degradation rate (25.76 ± 4.90 %). The drug release profile of CUR exhibited a consistent release rate of 57.0 ± 3.9 % during the initial phase over a 24-h period. The seeded L929 cells' behavior on the scaffolds was assessed using the MTT assay and FESEM microimages. The PCL/CS/CUR25μM showed no cytotoxic effect on the seeded cells, and they exhibited appropriate adherence to the scaffolds. Therefore, the electrospun core-shell PCL/CS/CUR25μM nanofibrous composite has the potential to function as an appropriate membrane for wound healing applications. © 2024 Elsevier Ltd
