Articles
World Journal of Microbiology and Biotechnology (09593993)41(5)
Antibiotic residues, as emerging micropollutants in surface water, threaten human health and ecosystems. The degradation of antibiotics is an essential process for safeguarding public health. This study identifies and optimizes a novel yeast strain for co-amoxiclav (AMC) degradation. Meyerozyma guilliermondii DF3 (Accession No. MZ477354) was isolated from soil using a basal mineral medium (BMM) supplemented with AMC. The strain degraded 75% of AMC within 72 h. Key culture conditions (temperature (20–35 °C), carbon sources (glucose/glycerol), inoculum size (1–5% v/v), and pH (5–9) were optimized via single-factor experiments and Box-Behnken design (BBD). Residual antibiotic levels were quantified by UV spectroscopy and HPLC. Optimal biodegradation occurred with glycerol/glucose, pH 6, and 1% inoculum (1 × 10⁸ cells/mL) at 30 °C. Response surface methodology (RSM) predicted complete degradation (100%) at pH 6.25, 1.02% inoculum, and 95.76 h, with pH being the most influential factor. These findings highlight M. guilliermondii DF3 as a promising candidate for AMC bioremediation in aquatic environments, advancing yeast-based strategies for water treatment. © The Author(s), under exclusive licence to Springer Nature B.V. 2025.
Food Bioscience (22124306)69
The edible filamentous fungus, Neurospora intermedia, has attracted significant attention over the past decade as a novel candidate for sustainable food and feed sources. This study aimed to evaluate the factors that enhance the content of B vitamins and carotenoid pigments in fungal biomass. We investigated the influence of physiological growth conditions, as well as other factors affecting the biosynthesis of these bioactive compounds, including precursors, metal cofactors, and stress conditions. Optimal conditions for biomass and carotenoid production were identified as 15 g/L glucose, 0.5 % (w/v) yeast extract, pH 6, and a 1 % (v/v) inoculum level in basic mineral medium. Among the metal cofactors tested, 5 mmol Mo2+ exhibited a highly significant effect on pigment production. The synthesis of B-group vitamins was influenced by disodium succinate after 72 h and 96 h, and by ribose after 72 h incubation. The maximum production levels recorded for biomass, carotenoids, and vitamins B1, B2, B3, and B6 were 4.90 g DM/L, 168.23 μg/g, 3.85, 12.49, 0.35, and 3.81 mg/g, respectively. This study highlights the biotechnological potential for producing biomass and bioactive compounds as viable resources for food and feed applications. © 2025 The Authors
