Endurance training and LXR activation increased LXRα and ABCA1 transcripts in Wistar male rats[L'entraînement en endurance et l'activation de LXR augmentent la transcription de LXRα et ABCA1 chez le rat]

Abstract

Purpose: LXR, belongs to the ligands activated transcription factors, regulates the expression of genes in cholesterol homeostasis. The aim of the present study was to examine combinatory effects of selective LXR activator-T0901317 and training on gene expression profiles of LXRα and ABCA1. Material and methods: To examine simultaneous effects of selective LXR activator-T0901317- and exercise on LXRα and ABCA1 genes expression and lipids profile, Wistar male rats were assigned to four groups as animal models: Control group (Ctrl), trained group received exercise (Exc) on a motor-driven treadmill at 28 meter/min for 60 min/day, 5 days/week for 8 weeks, group receiving LXR agonist T0901317 (10 mg kg−1 day−1) (Ago) for 1 week, group received both endurance training and LXR agonist T0901317 (Exc&Ago). Finally, rats were killed and blood was taken for lipids measurement. A portion of the liver was also excised for assessment of LXRα and ABCA1 transcripts. Results: A significant increase in LXRα and ABCA1 transcripts was observed in Exc&Ago group when compared to other groups. However, there was not a synergistic response in plasma lipid and lipoprotein concentrations despite a significant increase in LXRα and ABCA1 mRNA levels, possibly due to a physiological negative feedback loop, the response to exercise could be negated by the effects of the agonist in plasma lipid and lipoprotein profiles. © 2018 Elsevier Masson SAS