Possible Association of Glutathione S-Transferase Pi 1 (GSTP1) Gene Polymorphism in Iraqi Patients with Breast Cancer

Abstract

Background: Investigating the role of glutathione S-transferase pi 1 (GSTP1) in breast cancer development and exploring genetic variations in GSTP1 that may contribute to susceptibility to the disease. Methods: Blood samples were collected from 40 healthy control individuals and 75 breast cancer patients for genomic DNA extraction. PCR and bioinformatics analysis were used to examine the GSTP1 gene sequences. Results: Gel electrophoresis confirmed the presence of a 433-bp amplified genetic locus with a 212C>A variation in the intron-4 region, identified as rs757152293. The Single nucleotide polymorphisms (SNP) exhibited variable distributions, with homozygous CC and heterozygous CA genotypes. Patient samples with the CA genotype were submitted to NCBI under accession numbers OL957029-OL957036, while those with the CC genotype were submitted under accession numbers OL957027-OL957034. Additionally, sequence analysis of the rs757152293 SNP in healthy individuals was submitted to NCBI under accession numbers OL957037-OL957041. Conclusion: This study is the first to suggest a possible association between GSTP1 genetic polymorphism and breast cancer in the investigated population. © 2025 Varastegan Institute for Medical Sciences. All rights reserved.