دکتر فاتح رحیمی

دانشیار

گروه زیست‌شناسی سلولی مولکولی و میکروبیولوژی
دانشکده علوم و فناوری‌های زیستی

پست الکترونیکی

f.rahimi@sci.ui.ac.ir

تلفن

+983137932471

آدرس

دانشگاه اصفهان میدان آزادی اصفهان، ایران
کد پستی : 8174673441

لینک‌های وب

صفحه شخصی

معرفی کلی

درباره من

بنده به عنوان دانشیار باکتری شناسی در دانشگاه اصفهان در حال تحقیق بر روی بیماریزایی باکتریها و انتشار کلونال باکتریهای مقاوم به آنتی بیوتیک با تأکید بر باکتریهای مولد بیوفیلم هستم.

علاقه مندی پژوهشی

باکتری شناسی پزشکی و بیماریهای عفونی با تأکید بر: 1- عفونتهای دستگاه ادراری 2- عفونتهای زخم

عضویت در انجمن ها و گروههای تحقیقاتی

1- عضو انجمن میکروبشناسی بالینی و بیماریهای عفونی اروپا (ESCMID) 2- عضو پیوسته انجمن متخصصین بیماریهای عفونی و گرمسیری ایران 3- عضو پیوسته انجمن علمی باکتری شناسی پزشکی ایران

فعالیت‌های حرفه‌ای

1- مقاومت آنتی بیوتیکی و ترکیبات ضد میکروبی 2- اپیدمیولوژی مولکولی و تایپینگ باکتریها 3- بیماریزایی باکتریها و عوامل حدت 4- بیوفیلم باکتریها

سابقه تحصیلی

دکتر, دکتری تخصصی, دانشگاه اصفهان [اصفهان - ایران]
پسادکتری, محقق, انستیتو کارولینسکا [استکهلم - سوئد]

دروس

کارشناسی: 1- باکتری شناسی گرم مثبتها 2- مهندسی ژنتیک میکروب 3- میکروبیولوژی بیماریهای عفونی کارشناسی ارشد: 1- آنتی بیوتیکها و سازوکار عمل 2- باکتری شناسی پیشرفته 3- سموم میکروبی و سازوکار عمل 4- روشها در میکروبیولوژی 5- ژنتیک پروکاریوتها دکتری: 1- ژنتیک میکروبها 2- روشهای پیشرفته آزمایشگاهی در میکروبیولوژی 3- روابط انگل و میزبان 4- مباحث ویژه

برونداد‌های پژوهشی

همه موارد

مقالات

کتاب

مقالات لاتین

2025

Antibiotic-potentiating efficacy of Rosmarinus officinalis L. to combat planktonic cells, biofilms, and efflux pump activities of extensively drug-resistant Acinetobacter baumannii clinical strains

( Article . Gold )

Khashei, S., Fazeli, H., Rahimi, F., Karbasizade, V.

Publication Date: 2025

Frontiers in Pharmacology (16639812)16

Exploring the genetic diversity and the association of drug resistance and biofilm production in Acinetobacter baumannii strains isolated from burn wound infections

( Article . )

Khashei, S., Fazeli, H., Rahimi, F., Karbasizade, V.

Publication Date: 2025

Iranian Journal Of Microbiology (20083289)17(4)pp. 549-558

2024

Characterization of Clonal Groups of Antibiotic-Resistant Biofilm-Forming Staphylococcus aureus Strains Isolated from Patients with Urinary Tract Infections in Isfahan, Iran

( Article . Gold )

Mostafavi, N.S., Rahimi, F., Khashei, S.

Publication Date: 2024

Archives Of Clinical Infectious Diseases (23452641)19(4)

Background: Over the past decades, the role of biofilm-forming Staphylococcus aureus strains in urinary tract infections (UTIs) has garnered significant attention. Objectives: This study aimed to determine the epidemiological characteristics and diversity of S. aureus strains isolated from patients with UTIs in Isfahan, Iran, in 2017, with regard to their antimicrobial resistance, biofilm formation, and phylogenetic profiles. Additionally, the study investigated potential relationships among these factors statistically to develop efficient control and treatment approaches. Methods: All patients with symptomatic UTIs who had positive urine cultures for S. aureus during the study period at the laboratory of a referral hospital in Isfahan were included. All isolates were identified using specific primers for the nucA gene. Their biofilm formation capacity was evaluated using a combination of the microtiter plate and Congo-red agar methods. Antibiotic susceptibility testing was performed using the disk diffusion method. The presence of genes involved in biofilm formation and resistance to cefoxitin, aminoglycosides, and fluoroquinolones was detected using polymerase chain reaction (PCR). Staphylococcal cassette chromosome mec (SCCmec) typing, agr typing, and phene plate (PhP) typing were employed to investigate the diversity of collected strains. Results: Results showed that 19%, 57%, and 24% of confirmed S. aureus strains were strong, intermediate, and non-biofilm formers, respectively. The highest rate of resistance was against nalidixic acid (77%), followed by streptomycin (73%). The icaD and icaA genes had the highest frequency among biofilm-producing strains. gyrA (44%) and grlA (35%) were the most frequent genes among fluoroquinolone-resistant strains, while aph(3′)-IIIa was the most prevalent aminoglycoside-modifying enzyme gene. The majority of bacterial strains harbored SCCmec type III and agr type I. PhP typing of strains revealed the presence of 8 common types (CTs) and 14 single types (STs), with CT2 being the dominant type. Conclusions: The present investigation revealed various biofilm production capacities, antimicrobial resistance profiles, and clonal lineages in S. aureus isolated from patients with UTIs. These findings provide further insights into the epidemiology and pathogenicity of S. aureus strains in Iran, thereby improving the quality of surveillance and therapeutic protocols. © 2024, Mostafavi et al.

Investigation the effect of nitric oxide on biofilm-producing uropathogenic Escherichia coli strains isolated from patients

( Article )

Rahimi, F., Khashei, S.

Publication Date: 2024

Tehran University Medical Journal (16831764)82(7)pp. 528-537

Background: Biofilm producing uropathogenic Escherichia coli (UPEC) strains are of major concern in clinical settings which display increased resistance to conventional antimicrobial therapy. Nitric oxide (NO) has shown to exhibit anti-biofilm effects in a variety of bacterial species. In this study we aimed to evaluate the effectiveness of NO on the formation and eradication of biofilm of UPEC strains isolated from patients with urinary infection (UI) in Tehran. Methods: During May 2022 to April 2023, a total of 3814 suspected isolates of UPEC were collected from a pathobiology laboratory in Tehran and confirmed as E. coli strains using specific primers for elongation factor Tu (tufA) gene. All strains were screened for the ability to form biofilm by the microtiter plate (MTP) and Congo red agar (CRA) assays, and also the presence of biofilm genes were detected among biofilm producing strains. Moreover, the effect of NO on biofilm formation and its dispersal was evaluated by the high (30 mM) and low (125 nM) concentrations of sodium nitroprusside (SNP) as NO donor. Results: All collected isolates were confirmed by the polymerase chain reaction (PCR) using specific primers, in which 1309 strains (34%) were able to form colonies with red, dry and rough (rdar, curli and cellulose positive) (n=682, 52%), brown, dry and rough (bdar, curli positive and cellulose negative) (n=353, 27%) and pink, dry and rough (pdar, cellulose positive and curli negative) (n=274, 21%) morphotypes on CRA and selected as biofilm positive strains. Furthermore, 228 (17%), 402 (31%) and 679 (52%) strains were able to form a weak, moderate, and strong biofilm, respectively, and csgA, csgD, yedQ, and bcsA genes were found among 87, 98, 100 and 79% of biofilm-positive strains, respectively. The 30 mM concentration of SNP significantly decreased the biofilm formation (17-40%) and increased biofilm dispersal (20-45%) among UPEC strains. Conclusion: These findings confirmed the applicability of nitric oxide as an anti-biofilm agent for UPEC strains. These findings contribute to the development of novel strategies for fighting biofilm-associated infections. Copyright © 2024 Rahimi et al.

Investigation the effect of nitric oxide on biofilm-producing uropathogenic Escherichia coli strains isolated from patients[بررسی اثر اکسید نیتریک بر سویههای اشرشیا کلای اوروپاتوژنیک مولد بیوفیلم جدا شده از بیماران]

( Article )

Rahimi, F., Khashei, S.

Publication Date: 2024

Tehran University Medical Journal (17357322)82(7)pp. 528-537