دکتر مریم نوربخش نیا

Recent years, the rapid advancement of technology has raised concerns. We studied the effects of prenatal exposure to 900 MHz radiofrequency (RF) from mobile phones and the protective effects of linalool on learning and memory, and anxiety in adolescent male and female offspring rats. Pregnant rats were divided into four groups: control, wave, wave + linalool, and linalool. Rats received linalool (25mg/kg) by gavage for 21 days. Irradiation was conducted from day 0 to day 21 of pregnancy. Offsprings underwent behavioral and electrophysiological tests on days 50 and 60 after birth. Exposure to RF during pregnancy caused anxiety-like behavior in the EPM test and impairment of learning and memory in the Morris water maze and shuttle box tests. Electrophysiological properties and synaptic plasticity of the dorsal hippocampal CA3-CA1 synapse showed a decrease in fEPSP amplitude and slope. The trace element levels in both male and female offspring were consistent across all groups compared to their respective controls. In the hippocampus tissue, the levels of Fe, Cu, and Mn, as well as the Cu/Zn ratio, were significantly higher in the exposed groups (wave groups) compared to their controls. Moreover, Zn levels were significantly lower in the hippocampus tissue of the exposed groups. Linalool administration mitigated the excessive increase in Fe, Cu, Mn, and Cu/Zn ratio and normalized the disrupted levels of trace elements, except for Zn levels in both male and female offspring. Sex differences were observed in the EPM and shuttle box tests, females were more sensitive than males. In summary, our study demonstrates that prenatal exposure to mobile phone radiation induces stress-like behaviors, disrupts learning and memory, alters hippocampal electrophysiological properties and trace element balance in offspring. Treatment with linalool mitigates these deleterious effects, highlighting its potential as a therapeutic intervention. These findings contribute to our understanding of the impact of prenatal environmental exposures on neurodevelopment and offer insights into potential strategies for neuroprotection. © The Author(s) 2024.

Protective effects of a lactobacilli mixture against Alzheimer’s disease-like pathology triggered by Porphyromonas gingivalis

( Article . Gold )

Kazemi, N., Rabbani, M., Noorbakhshnia, M., Razavi, S.M., Narimani, T., Naghsh, N.

Scientific Reports (20452322)14(1)

Porphyromonas gingivalis (P. gingivalis) is one of the pathogens involved in gingival inflammation, which may trigger neuroinflammatory diseases such as Alzheimer’s disease (AD). This study aimed to investigate the protective (preventive and treatment) effects of a lactobacilli mixture combining Lactobacillus reuteri PTCC1655, Lactobacillus brevis CD0817, Lacticaseibacillus rhamnosus PTCC1637, and Lactobacillus plantarum PTCC1058 against P. gingivalis-induced gingival inflammation and AD-like pathology in rats. These probiotic strains exhibited cognitive enhancement effects, but this study proposed to assess their activity in a mixture. To propose a probable mechanism for P. gingivalis cognitive impairments, the TEs balance were analyzed in hippocampus and cortex tissues. Animals were divided into five groups: the control, lactobacilli, P. gingivalis, lactobacilli + P. gingivalis (prevention), and P. gingivalis + lactobacilli group (treatment) groups. The behavioral and histopathological changes were compared among them. Finally, The Trace elements (TEs) levels in the hippocampus and cortex tissues were analyzed. The palatal tissue sections of the P. gingivalis infected rats showed moderate inflammation with dense infiltration of inflammatory cells, a limited area of tissue edema, and vascular congestion. Additionally, passive avoidance learning and spatial memory were impaired. Histopathological tests revealed the presence of Aβ-positive cells in the P. gingivalis group. While the Aβ-positive cells decreased in the treatment group, their formation was inhibited in the preventive group. Administration of a mixture of lactobacilli (orally) effectively mitigated the gingival inflammation, Aβ production, and improved learning and memory functions. Moreover, Zn, Cu, and Mn levels in the hippocampus were dramatically elevated by P. gingivalis infection, whereas lactobacilli mixture mitigated these disruptive effects. The lactobacilli mixture significantly prevented the disruptive effects of P. gingivalis on gingival and brain tissues in rats. Therefore, new formulated combination of lactobacilli may be a good candidate for inhibiting the P. gingivalis infection and its subsequent cognitive effects. The current study aimed to evaluate the effects of a lactobacilli mixture to manage the disruptive effects of P. gingivalis infection on memory. © The Author(s) 2024.

2023

The synergic effects of presynaptic calcium channel antagonists purified from spiders on memory elimination of glutamate-induced excitotoxicity in the rat hippocampus trisynaptic circuit

( Article . Gold )

Keimasi, M., Salehifard, K., Mirshah jafar esfahani, N., Esmaeili, F., Farghadani, A., Amirsadri, M., Keimasi, M., Noorbakhshnia, M., Moradmand, M., Mofid, M.R.

Frontiers in Molecular Biosciences (2296889X)10

The hippocampus is a complex area of the mammalian brain and is responsible for learning and memory. The trisynaptic circuit engages with explicit memory. Hippocampal neurons express two types of presynaptic voltage-gated calcium channels (VGCCs) comprising N and P/Q-types. These VGCCs play a vital role in the release of neurotransmitters from presynaptic neurons. The chief excitatory neurotransmitter at these synapses is glutamate. Glutamate has an essential function in learning and memory under normal conditions. The release of neurotransmitters depends on the activity of presynaptic VGCCs. Excessive glutamate activity, due to either excessive release or insufficient uptake from the synapse, leads to a condition called excitotoxicity. This pathological state is common among all neurodegenerative disorders, such as Alzheimer’s and Parkinson’s diseases. Under these conditions, glutamate adversely affects the trisynaptic circuitry, leading to synaptic destruction and loss of memory and learning performance. This study attempts to clarify the role of presynaptic VGCCs in memory performance and reveals that modulating the activity of presynaptic calcium channels in the trisynaptic pathway can regulate the excitotoxic state and consequently prevent the elimination of neurons and synaptic degradation. All of these can lead to an improvement in learning and memory function. In the current study, two calcium channel blockers—omega-agatoxin-Aa2a and omega-Lsp-IA—were extracted, purified, and identified from spiders (Agelena orientalis and Hogna radiata) and used to modulate N and P/Q VGCCs. The effect of omega-agatoxin-Aa2a and omega-Lsp-IA on glutamate-induced excitotoxicity in rats was evaluated using the Morris water maze task as a behavioral test. The local expression of synaptophysin (SYN) was visualized for synaptic quantification using an immunofluorescence assay. The electrophysiological amplitudes of the field excitatory postsynaptic potentials (fEPSPs) in the input-output and LTP curves of the mossy fiber and Schaffer collateral circuits were recorded. The results of our study demonstrated that N and P/Q VGCC modulation in the hippocampus trisynaptic circuit of rats with glutamate-induced excitotoxicity dysfunction could prevent the destructive consequences of excitotoxicity in synapses and improve memory function and performance. Copyright © 2023 Keimasi, Salehifard, Mirshah Jafar Esfahani, Esmaeili, Farghadani, Amirsadri, Keimasi, Noorbakhshnia, Moradmand and Mofid.

2021

The effects of lactobacilli (L. rhamnosus, L. reuteri, L. Plantarum) on LPS-induced memory impairment and changes in CaMKII-α and TNF-α genes expression in the hippocampus of rat

( Article . )

Zolfaghari, S.I., Rabbani, M., Noorbakhshnia, M.

Physiology and Behavior (1873507X)229

2020

Lidocaine Reversible Inactivation of the Central Nucleus of Amygdala Impairs Acquisition, Consolidation, and Retrieval of Morphine State-Dependent Learning in Rat

( Article . )

Noorbakhshnia, M., Rahmani-samani, P., Eivani, M.

Pharmacology (00317012)105(3-4)pp. 225-230

Quercetin-Conjugated Superparamagnetic Iron Oxide Nanoparticles Protect AlCl3-Induced Neurotoxicity in a Rat Model of Alzheimer’s Disease via Antioxidant Genes, APP Gene, and miRNA-101

( Article . Gold • Green )

Amanzadeh, E., Esmaeili, A., Rahgozar, S., Noorbakhshnia, M.

Frontiers in Neuroscience (1662453X)14

2019

The role of nucleus accumbens shell on acquisition and retrieval stages of morphine state dependent learning

( Article . )

Noorbakhshnia, M., Zarrinimehr, N.

Asian Journal of Psychiatry (18762018)39pp. 150-156

2018

Agmatine attenuates methamphetamine-induced passive avoidance learning and memory and CaMKII-α gene expression deteriorations in hippocampus of rat

( Article . )

Noorbakhshnia, M., Rashidkaboli, A., Pakatchian, M., Beheshti, S.

Physiology and Behavior (1873507X)194pp. 491-496

Alteration in messenger RNAneurotrophin 4 and tyrosine kinase receptors B expression levels following spinal cord injury

( Article . )

Esmaeili, A., Pakravan, G., Ghaedi, K., Noorbakhshnia, M.

Journal of Neurosurgical Sciences (03905616)62(2)pp. 146-152

2017

Portulaca oleracea L. prevents lipopolysaccharide-induced passive avoidance learning and memory and TNF-α impairments in hippocampus of rat

( Article . )

Noorbakhshnia, M., Karimi-zandi, L.

Physiology and Behavior (1873507X)169pp. 69-73

2016

Nurr1 and PPARγ protect PC12 cells against MPP+ toxicity: involvement of selective genes, anti-inflammatory, ROS generation, and antimitochondrial impairment

( Article . )

Jodeiri farshbaf, M., Forouzanfar, M., Ghaedi, K., Kiani-esfahani, A., Peymani, M., Shoaraye nejati, A., Izadi, T., Karbalaie, K., Noorbakhshnia, M., Rahgozar, S.

Molecular And Cellular Biochemistry (15734919)420(1-2)pp. 29-42

2015

Effect of olibanum on a rat model of Alzheimer’s disease induced by intracerebroventricular injection of streptozotocin

( Article )

Zaker, S.R., Beheshti, S., Aghaie, R., Noorbakhshnia, M.

Physiology and Pharmacology (17350581)18(4)pp. 477-489

Omega-3 fatty acids prevent LPS-induced passive avoidance learning and memory and CaMKII-α gene expression impairments in hippocampus of rat

( Article . )

Noorbakhshnia, M., Dehkordi, N.G., Ghaedi, K., Esmaeili, A., Dabaghi, M.

Pharmacological Reports (17341140)67(2)pp. 370-375

2014

Role of hippocampal CA1 area gap junction channels on morphine state-dependent learning

( Article . )

Beheshti, S., Hosseini, S.A.M.S., Noorbakhshnia, M., Eivani, M.

European Journal of Pharmacology (00142999)745pp. 196-200

2011

Effect of culture condition and cell-permeable superoxide dismutase on levels of reactive oxygen species (ROS) production in " in vitro" produced sheep embryos

( Article . )

Hosseini, S.O., Aghaee f., , Hosseini s.m., , Hajian m., , Forouzanfar m., , Noorbakhshnia, M., Gourabi h., , Shahverdi a.h., , Vosough taghi dizaj a., , Nasr-esfahani, M.H.

Small Ruminant Research (09214488)97(1-3)pp. 88-93

Orexin-1 receptor mediates long-term potentiation in the dentate gyrus area of freely moving rats

( Article . )

Akbari, E., Motamedi, F., Davoodi, F.G., Noorbakhshnia, M., Ghanbarian, E.

Behavioural Brain Research (18727549)216(1)pp. 375-380

2010

Copines-1, -2, -3, -6 and -7 show different calcium-dependent intracellular membrane translocation and targeting

( Article . Bronze )

Perestenko, P.V., Pooler, A.M., Noorbakhshnia, M., Gray, A., Bauccio, C., Jeffrey mcilhinney, R.A.

FEBS Journal (1742464X)277(24)pp. 5174-5189

2008

The effect of antagonization of orexin 1 receptors in CA1 and dentate gyrus regions on memory processing in passive avoidance task

( Article . )

Akbari, E., Motamedi, F., Naghdi, N., Noorbakhshnia, M.

Behavioural Brain Research (18727549)187(1)pp. 172-177

2006

Effect of the GABAergic system on memory formation and state-dependent learning induced by morphine in rats

( Article . )

Zarrindast, M., Noorbakhshnia, M., Motamedi, F., Haeri rohani a., A., Rezayof, A.

Pharmacology (00317012)76(2)pp. 93-100

2004

Involvement of nucleus accumbens in L-arginine-induced conditioned place preference in rats

( Article . )

Sahraei h., , Pirzadeh-jahromi g., , Noorbakhshnia, M., Asgari a., , Haeri rohani a., A., Khoshbaten a., , Poorheidari gh.r., , Sepehri, H., Ghoshooni h., , Zarrindast, M.

Behavioural Pharmacology (14735849)15(7)pp. 473-480