Publication Date: 2025
Iranian Journal Of Science (27318095)49(3)pp. 577-584
Fluorescent carbon dots (CDs) have attracted great attention for the biomedical applications as a new group of nanoparticles with high biocompatibility and interesting optical properties. In this study, the fluorescent CDs were synthesized by a biogenic hydrothermal method using Taxus baccata extract and ethylene diamine (EDA). The CDs, predominantly semi-spherical and smaller than 20 nm, exhibit high colloidal stability. They have a hydrodynamic size of 43.6 nm, a polydispersity index (PI) of 0.526, and a zeta potential of -56.7 mV. Their surface is rich in hydroxyl and carbonyl groups. They also exhibit fluorescence emission, with a quantum yield (QY) of 11.6% at 490 nm, when excited at 400 nm. The in vitro cytotoxicity assay on the human fibroblast (HFB) and umbilical vein endothelial (HUVEC) cells confirmed the biocompatibility of CDs. These CDs represent significant antibacterial effects on Staphylococcus aureus and Escherichia coli with the minimum inhibitory concentrations (MIC) of 40 and 160 μg mL− 1, respectively. The rapid and efficient uptake of CDs with E. coli and HFB cells was confirmed by the fluorescence imaging. Based on the results, the biogenic CDs can be a suitable alternative to the antibiotics against pathogenic bacteria and also for the staining and labeling of the microbial cells. © The Author(s), under exclusive licence to Shiraz University 2024.
Publication Date: 2025
Scientific Reports (20452322)15(1)
Efficient encapsulation of bioactive compounds in the food-grade delivery systems to improve their stability, efficiency, and bioavailability and to develop functional foods is a novel and interesting field of research in food industry. Here, two unstable and hydrophobic food ingredients including vitamin E (Vit E) and clove essential oil (CEO) were encapsulated in the chitosan nanoparticles (ChNPs) by the emulsion/ ionic gelation technique without using any toxic surfactant. The physicochemical characterization by DLS, FTIR, FE-SEM, and TEM, confirmed the synthesis of high colloidal and monodispersed ChNPs with narrow size distribution of less than 50 nm. MTT assay showed high biocompatibility of ChNPs after CEO loading, with the increased cellular viability of 72.84% for CEO loaded ChNPs. ABTS and DPPH assays also revealed high antioxidant activity of CEO loaded ChNPs with maximum activity of 75.66%. Co-loading of Vit E and CEO led to the antioxidant activity of 77.7% over 48 h, potent antibacterial effect on E. coli and S. aureus with the inhibition zone diameters of 14–17 mm, and complete growth inhibition of fungus A. niger. Molecular docking studies also showed strong binding of chitosan, eugenol, and α-tocopherol to different bacterial and fungal proteins. Overall results indicated that Vit E and CEO loaded ChNPs represent interesting physicochemical and biological properties and high potential for development of food preservatives and functional foods. © The Author(s) 2025.
Publication Date: 2024
Journal of Biotechnology (01681656)387pp. 23-31
Enzyme immobilization in membrane bioreactors has been considered as a practical approach to enhance the stability, reusability, and efficiency of enzymes. In this particular study, a new type of hybrid membrane reactor was created through the phase inversion method, utilizing hybrid of graphene oxide nanosheets (GON) and polyether sulfone (PES) in order to covalently immobilize the Candida rugosa lipase (CRL). The surface of hybrid membrane was initially modified by (3-Aminopropyl) triethoxysilane (APTES), before the use of glutaraldehyde (GLU), as a linker, through the imine bonds. The resulted enzymatic hybrid membrane reactors (EHMRs) were then thoroughly analyzed by using field-emission scanning electron microscopy (FE-SEM), contact angle goniometry, surface free energy analysis, X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, attenuated total reflection (ATR), and energy-dispersive X-ray (EDX) spectroscopy. The study also looked into the impact of factors such as initial CRL concentration, storage conditions, and immobilization time on the EHMR's performance and activity, which were subsequently optimized. The results demonstrated that the CRLs covalently immobilized on the EHMRs displayed enhanced pH and thermal stability compared to those physically immobilized or free. These covalently immobilized CRLs could maintain over 60% of their activity even after 6 reaction cycles spanning 50 days. EHMRs are valuable biocatalysts in developing various industrial, environmental, and analytical processes. © 2024 Elsevier B.V.
Publication Date: 2024
RSC Advances (20462069)14(24)pp. 16801-16808
Cobalt ferrite (CoFe) nanoparticles (NPs) with appropriate physicochemical and biological properties have attracted great attention for biomedical applications. In the present study, chitosan-coated mesoporous CoFe (CoFeCH) NPs were synthesized using a facile one-step hydrothermal method and fully characterized using FE-SEM, EDS, BET, FTIR spectroscopy, DLS, TGA, XRD, and VSM. The spherical, highly colloidal, and monodispersed CoFeCH NPs with an average hydrodynamic size of 177.9 nm, PDI of 0.238 and zeta potential value of −33 represented a high saturation magnetization value of 59.37 emu g−1. N2 adsorption-desorption analysis confirmed the mesoporous structure of CoFeCH NPs with a type IV isotherm, calculated specific surface area of 89.583 m2 g−1 and total pore volume of 0.3668 cm3 g−1. CoFeCH NPs exhibited high antibacterial effects on S. aureus and E. coli, comparable with standard antibiotics, while CH-coating led to higher biocompatibility of CoFe NPs on human cells in vitro. CoFeCH NPs also showed significant peroxidase activity with a Km value of 14.37 and specific activity of 0.632 mmol min−1. CoFeCH NPs were successfully used as a MRI contrast agent with an R2 value of 91.3 mM−1 s−1. The overall results indicated the high potential of synthesized CoFeCH NPs by the present method for biomedical applications, especially as an antibacterial and MRI contrast agent. © 2024 The Royal Society of Chemistry.
Publication Date: 2024
Scientific Reports (20452322)14(1)
A simple, one-pot and green method is reported for hydrothermal synthesis of highly fluorescent and magnetic carbon dots (CDs) by using D-glucose, as the carbon source. CDs were fully characterized by the UV-Vis and fluorescence spectroscopy, DLS, FTIR, TEM, EDS, XRD, and VSM. The nitrogen doping of different diamines significantly improved the fluorescence quantum yield (QY) of CDs with the maximum effect obtained by using m-phenylenediamine (mPDA). Temperature and reaction time also affected the QY of CDs with the best results obtained at 150 °C for 3 h. The heteroatom doping by innovative use of different metal sulfates including FeSO4, MnSO4, CuSO4, MgSO4, and ZnSO4, further improved the optical properties of CDs. Interestingly, the magnetic and multicolor CDs with high colloidal stability and QYs of 17.7, 16.5, and 53.9% at 460, 490, and 515 nm, respectively, were synthesized by using 0.1 M of glucose, mPDA and MnSO4. The resulted Mn-, S-, N-doped CDs represented rapid uptake and high-quality fluorescence imaging of the human fibroblast and umbilical vein endothelial cells in vitro, without significant toxicity. The CDs also displayed high r1 relaxivity of 32.3 mM− 1 s− 1 and were used for high-contrast MR and fluorescence imaging of mouse tumor models, in vivo. © The Author(s) 2024.
Publication Date: 2023
RSC Advances (20462069)13(14)pp. 9491-9500
A new and efficient theranostic nanoplatform was developed via a green approach for targeted cancer therapy and fluorescence imaging, without the use of any anticancer chemotherapeutic drugs. Toward this aim, monodisperse and spherical mesoporous silica nanoparticles (MSNs) of approximately 50 nm diameter were first synthesized using the sol-gel method and loaded with hydrothermally synthesized anticancer carbon dots (CDs). The resulting MSNs-CDs were then functionalized with chitosan and targeted by an anti-MUC1 aptamer, using the glutaraldehyde cross-linker, and fully characterized by TEM, FE-SEM, EDS, FTIR, TGA, XRD, and BET analysis. Potent and selective anticancer activity was obtained against MCF-7 and MDA-MB-231 cancer cells with the maximum cell mortalities of 66.2 ± 1.97 and 71.8 ± 3%, respectively, after 48 h exposure with 100 μg mL−1 of the functionalized MSNs-CDs. The maximum mortality of 40.66 ± 1.3% of normal HUVEC cells was obtained under the same conditions. Based on the results of flowcytometry analysis, the apoptotic mediated cell death was recognized as the main anticancer mechanism of the MSNs-CDs. The fluorescence imaging of MCF-7 cancer cells was also studied after exposure with MSNs-CDs. The overall results indicated the high potential of the developed nanoplatform for targeted cancer theranostics. © 2023 The Royal Society of Chemistry.
Publication Date: 2022
Scientific Reports (20452322)12(1)
The aim of this study is to fabricate a new scaffold appropriate for tissue regeneration with antimicrobial activity and ability of controlled drug delivery. In this regard, scaffold nanofibers were produced using poly (methyl methacrylate) (PMMA), Mo132 as a Keplerate polyoxometalate and metronidazole. The final scaffolds, obtained by electrospinning, represent the intrinsic features including exceptional doubling tensile strength, high hydrophilicity (126 ± 5.2° to 83.9 ± 3.2° for contact angle and 14.18 ± 0.62% to 35.62 ± 0.24% for water uptake), proper bioactivity and cell adhesion. Moreover, the addition of Mo132 and metronidazole enhances the biodegradation rate of resulted scaffolds compared to the pure PMMA membrane. The controlled release of metronidazole over 14 days efficiently inhibits the colonization of anaerobic microorganisms. Overall, the results demonstrate high potential of Mo132 and metronidazole-loaded PMMA scaffold for guided bone regeneration/guided tissue regeneration. © 2022, The Author(s).
Publication Date: 2022
RSC Advances (20462069)12(51)pp. 32834-32843
Facile and sensitive detection and isolation of circulating tumor cells (CTCs) was achieved using the aptamer-targeted magnetic nanoparticles (Apt-MNPs) in conjugation with a microfluidic device. Apt-MNPs were developed by the covalent attachment of anti-MUC1 aptamer to the silica-coated magnetic nanoparticles via the glutaraldehyde linkers. Apt-MNPs displayed high stability and functionality after 6 months of storage at 4 °C. The specific microfluidic device consisting of mixing, sorting and separation modules was fabricated through conventional photo- and soft-lithography by using polydimethylsiloxane. The capture efficiency of Apt-MNPs was first studied in vitro on MCF-7 and MDA-MB-231 cancer cell lines in the bulk and microfluidic platforms. The cell capture yields of more than 91% were obtained at the optimum condition after 60 minutes of exposure to 50 μg mL−1 Apt-MNPs with 10 to 106 cancer cells in different media. CTCs were also isolated efficiently from the blood samples of breast cancer patients and successfully propagated in vitro. The isolated CTCs were further characterized using immunofluorescence staining. The overall results indicated the high potential of the present method for the detection and capture of CTCs. © 2022 The Royal Society of Chemistry.
Publication Date: 2022
Journal of Drug Delivery Science and Technology (17732247)69
Wound healing is crucial because it entails a complex cellular process that complicates the design of scaffolds for therapeutic purposes. Present study is focused on the development of a new wound dressing based on polymethyl methacrylate nanofibers containing Mo72Fe30 as a Keplerate-type polyoxometalate with efficient and controlled delivery of metronidazole during wound healing. The hydrophilic nature of biocompatible Mo72Fe30 along with the antibiotic and wound healing properties of metronidazole were utilized to develop an efficient wound dressing. The efficient and controlled release of metronidazole was assisted by the presence of Keplerete as an anion and metronidazole as a cation. The nanofibers were prepared by electrospinning of appropriate solution and characterized by FT-IR, XRD, FE-SEM, EDX, TGA, and elemental analysis. The surface wettability, microstructure, water absorption capacity, mechanical properties, in-vitro biocompatibility, drug release behavior, and in-vivo wound healing of prepared dressings were also evaluated. The in-vivo animal studies (injury created on the back of Wistar rats) showed the complete healing of wounds after 14 days of being covered by wound dressing compared to a sterile gauze, which shows approximately 45% reduction in wound size. The amounts of wound closure using dressing were found to be 95.26 and 97.97% after 7 and 14 days of wounding, respectively. The overall results indicated the high potential of these nanofiber-based dressings for wound healing. © 2022 Elsevier B.V.
Publication Date: 2021
Dalton Transactions (14779226)50(11)pp. 3990-4007
To investigate the effect of different halogen substituents and leaving groups and the flexibility of ligands on the anticancer activity of copper complexes, sixteen copper(ii) complexes with eight different tridentate Schiff-base ligands containing pyridine and 3,5-halogen-substituted phenol moieties were synthesized and characterized by spectroscopic methods. Four of these complexes were also characterized by X-ray crystallography. The cytotoxicity of the complexes was determined in three different tumor cell lines (i.e.the A2780 ovarian, HCT116 colorectal and MCF7 breast cancer cell line) and in a normal primary fibroblast cell line. Complexes were demonstrated to induce a higher loss of cell viability in the ovarian carcinoma cell line (A2780) with respect to the other two tumor cell lines, and therefore the biological mechanisms underlying this loss of viability were further investigated. Complexes with ligandL1(containing a 2-pycolylamine-type motif) were more cytotoxic than complexes withL2(containing a 2-(2-pyridyl)ethylamine-type motif). The loss of cell viability in A2780 tumor cells was observed in the orderCu(Cl2-L1)NO3>Cu(Cl2-L1)Cl>Cu(Br2-L1)Cl>Cu(BrCl-L1)Cl. All complexes were able to induce reactive oxygen species (ROS) that could be related to the loss of cell viability. ComplexesCu(BrCl-L1)ClandCu(Cl2-L1)NO3were able to promote A2780 cell apoptosis and autophagy and for complexCu(BrCl-L1)Clthe increase in apoptosis was due to the intrinsic pathway.Cu(Cl2-L1)ClandCu(Br2-L1)Clcomplexes lead to cellular detachment allowing to correlate with the results of loss of cell viability. Despite the ability of theCu(BrCl-L1)Clcomplex to induce programmed cell death in A2780 cells, its therapeutic window turned out to be low making theCu(Cl2-L1)NO3complex the most promising candidate for additional biological applications. © The Royal Society of Chemistry 2021.
Publication Date: 2021
Nanomedicine (17486963)16(8)pp. 627-640
Aim: To develop a novel theranostic nanoplatform for simultaneous fluorescent monitoring and stimuli-triggered drug delivery. Materials & methods: Different microscopic and spectroscopic techniques were used for the characterization of nanocarriers. MCF-7 and human umbilical vein endothelial cell lines were cultured and treated with different doses of doxorubicin-loaded nanocarriers. The cell viability and drug release were studied using MTT assay and fluorescence microscopy. Results: Biocompatible and mono-disperse nanocarriers represent hollow and mesoporous structures with the calculated surface area of 552.83 m2.g-1, high magnetic activity (12.6 emu.g-1), appropriate colloidal stability and high drug loading capacity (up to 61%). Conclusion: Taxane-based carbon dots act as the pH-responsive gatekeepers for the controlled release of doxorubicin into cancer cells and provide a fluorescence resonance energy transfer system for real-time monitoring of drug delivery. © 2021 Future Medicine Ltd.
Publication Date: 2021
Polyhedron (02775387)210
A new hybrid compound was synthesized via the covalent grafting of vanadium-substituted Wells-Dawson type polyoxometalate (Bu4N)5H4[P2W15V3O62] to folic acid. The cytotoxic effect of the resulted bioactive hybrid compound was investigated on cervical (HeLa) and human breast (MCF-7) cancer cells. Interestingly this smart hybrid compound could knockdown cancer cells (IC50 about 19 and 75 µgmL−1 for HeLa and MCF-7 respectively), while the normal cells, as a folate receptor (FR) negative cell, remained nearly untreated (IC50 about 317 µgmL−1). Higher cytotoxicity of this hybrid polyoxometalate on HeLa than MCF-7 cells could be attributed to the higher expression of folic acid on HeLa. The IC50 for (Bu4N)5H4[P2W15V3O62] on healthy cells (about 84 µgmL−1) showed four times improvement in the polyoxometalate biocompatibility by incorporation of folic acid. In comparison to methotrexate (as a positive control), this smart polyoxometalate-folic acid hybrid compound has a high potential for developing a significant and selective therapeutic effect against FR-positive cancer cells. © 2021 Elsevier Ltd
Publication Date: 2021
Journal of Biotechnology (01681656)331pp. 26-36
The development of efficient drug nanocarriers has remained an important challenge in advanced drug delivery in human body. Combination of graphene-based nanomaterials and cyanuric chloride (CC), as a linker, may improve the success of drug delivery. Herein, a simple approach was used for the synthesis of superparamagnetic graphene oxide (SPMGO) nanocomposite through a chemical precipitation method. The nanocomposite was readily functionalized with cyanuric chloride as a linker for loading the drug. The FTIR spectroscopy confirmed the efficient synthesis of nanocarriers. So did the transmission electron microscopy, atomic force microscopy, and thermo-gravimetric analysis, X-ray diffraction and X-ray photoelectron spectroscopy. Subsequently, the synthesized nanocarriers were studied in terms of their potential for biomedical applications. Immobilization of methotrexate (MTX), as a drug for treatment of cancer was taken into action on the SPMGO and SPMGO/CC. The in vitro assays indicated that the drug nanocarrier systems, SPMGO/MTX and SPMGO/CC/MTX, are hemo-compatible and increase the efficiency of MTX against Caov-4, HeLa and MCF-7 cell lines. The MTX nanocarriers represented a considerably high drug loading and controlled drug release. The overall results indicated the great potential of SPMGO/CC/MTX nanocarrier for targeted drug delivery, particularly in MTX chemotherapy. © 2021
Publication Date: 2021
Informatics in Medicine Unlocked (23529148)25
The molecular nature, antioxidant, and anticancer actions of the dietary flavonoid daidzein binding to two types of β-lactoglobulin (βLG) are investigated in this work by using different kinds of spectroscopic techniques, electrochemical analysis, molecular docking, and molecular dynamics simulation. The results showed that hydrogen bonds and van der Waals interactions played a major role in the development of the stable complex. It may be deduced from molecular docking studies and competitive assays that daidzein binds to the outer surface of βLG with appropriate binding energies. The results of molecular dynamics showed the creation of a relatively stable complex in which daidzein binding reduced the flexibility of key residues in the binding site. In terms of the masking effect of βLG, the best antioxidant activity was found at low concentrations of the βLG-daidzein complex. After 48 h of exposure to a 50 μM mixture of βLG and daidzein, the complexes showed increased anticancer activity with maximal mortalities of 38.62 and 47.08% for HeLa and MCF-7 cells, respectively. The improvement of daidzein's solubility and anticancer activity as a result of its complexation with βLG, as well as its light and heat stability, could be a beneficial approach in the food and pharmaceutical industries. © 2021
Publication Date: 2021
ACS Applied Bio Materials (25766422)4(8)pp. 5908-5925
Cancer is considered one of the leading causes of death, with a growing number of cases worldwide. However, the early diagnosis and efficient therapy of cancer have remained a critical challenge. The emergence of nanomedicine has opened up a promising window to address the drawbacks of cancer detection and treatment. A wide range of engineered nanomaterials and nanoplatforms with different shapes, sizes, and composition has been developed for various biomedical applications. Nanomaterials have been increasingly used in various applications in bioimaging, diagnosis, and therapy of cancers. Recently, numerous multifunctional and smart nanoparticles with the ability of simultaneous diagnosis and targeted cancer therapy have been reported. The multidisciplinary attempts led to the development of several exciting clinically approved nanotherapeutics. The nanobased materials and devices have also been used extensively to develop point-of-care and highly sensitive methods of cancer detection. In this review article, the most significant achievements and latest advances in the nanomaterials development for cancer nanomedicine are critically discussed. In addition, the future perspectives of this field are evaluated. © 2021 American Chemical Society.
Publication Date: 2019
Journal of Hazardous Materials (03043894)366pp. 268-274
A comprehensive study was conducted toward the green and facile synthesis of biocompatible magnetite nanoparticles for the efficient removal of organic contaminants from water. The nanoparticles were synthesized using a modified co-precipitation method and functionalized by the taxane diterpenoids extracted from Taxus baccata L., and fully characterized using UV–vis spectroscopy, SEM, FTIR, VSM, and XRD. The synthesized monodisperse magnetite nanoparticles, with a narrow size distribution of less than 50 nm, displayed significant and stable magnetic activity without surface oxidation after several months. The batch experiments clearly indicated the efficient iron-catalyzed removal of Nile blue, methylene blue, methylene orange, and 4-nitrophenol for several cycles without significant loss of catalytic activity. The relevant kinetic data of the dyes removal reactions were fitted to a pseudo-first order model. Moreover, in vitro MTT assay revealed high biocompatibility of the nanoparticles with no significant toxicity on different human cell lines. The overall results indicated high potential of green synthesized, biocompatible magnetite nanoparticles for the environmental applications especially wastewater remediation. © 2018 Elsevier B.V.
Amiri rudbari, H.,
Mirkhani, V.,
Moghadam, M.,
Tangestaninejad, S.,
Mohammadpoor baltork, I.,
Abbasi kajani, A. Publication Date: 2019
Polyhedron (02775387)170pp. 70-85
A diastereomeric pair (homochiral and heterochiral) of Schiff base dioxo molybdenum complex was synthesized using chiral ligand (HL: R/S-1-((naphtalen-3-yl)methylenamino)propan-2-ol) in racemic form. Then, to separation of this diastereomeric pair, crystallization method was proposed which was successful with acetonitrile. 1H NMR technique was used to confirm the results. The complexes thoroughly characterized using FT-IR, elemental analysis, 1H NMR and 13C NMR techniques, and MoO2L(LH2)1 has been structurally characterized using single-crystal X-ray diffraction. The cytotoxic activity of the new compounds has been evaluated using MCF-7 (human breast adenocarcinoma) and HeLa (human cervix adenocarcinoma), in addition to normal human fibroblast cells using the MTT cytotoxicity assay. Compounds MoO2L(LH2)1 and MoO2L(LH2)2 revealed IC50 values 18 µM and 37 µM on MCF-7 and 58 µM and 17 µM on HeLa, respectively. MoO2L(LH2)2 showed high selectivity (3–13 folds) for cancerous cells over normal cells, as the maximum cell mortality of 8.77%. Furthermore, The HSA- and DNA-binding of the Mo(VI) complexes were investigated by absorption, emission spectroscopy, viscosity measurements and molecular docking. Their binding constant are calculated as: HSA-binding of MoO2L(LH2)1 is 6.7 × 104 M−1 and of MoO2L(LH2)2 is 5.8 × 104 M−1, while their DNA-binding are 5.6 × 104 M−1 and 2.2 × 105 M−1, respectively. Their thermodynamic parameters were also determined at different temperatures. © 2019 Elsevier Ltd
Publication Date: 2019
Journal of Biotechnology (01681656)298pp. 88-97
Polymer-coated nanocarriers play an important role in targeted drug delivery. The use of polymers such as polyethylene glycol increases stability, biocompatibility, and blood circulation time of the drug, and may consequently improve the success of drug delivery. In the present work, a simple approach has been reported for synthesizing polyethylene glycol bis amin (PEGA) functionalized graphene oxide/iron oxide nanocomposite as a remarkable unit for loading drugs. The biomedical applications of the synthesized nanocomposite were investigated by immobilizing methotrexate (MTX), as an anticancer drug. The structural and morphological characteristics and the successful synthesis of the nanocomposite were evaluated by different charachterization techniques. The cytotoxicity assay of the nanocarrier showed higher toxicity against HeLa and MCF-7 cell lines, compared to free MTX. The drug release experiments in acidic and physiological conditions suggested the first order kinetics model for the release of MTX from the nanocomposite. Furthermore, the agglutination, complement activation, and coagulation time experiments demonstrated the blood compatibility of the synthesized nanocarrier. © 2019 Elsevier B.V.
Publication Date: 2019
Polyhedron (02775387)160pp. 130-138
Reaction of 2-pyridinecarboxaldehyde with L-Alaninol (as a chiral amine) or 2-amino-2-methyl-1-propanol (AMP) in the presence of PdCl2 produced the new and attractive palladium(II) complexes which were synthesized by in situ method. The presence of an extra methyl group in AMP other than L-Alaninol induced the different chelating mode and, therefore, several structurally different palladium complexes obtained. Correspondingly, the chiral amine with PdCl2 produced the imine product, (Iminol (1)), and an oxazolidine compound (Imizol (1′)) as minor product. Also, the use of AMP in formation of palladium complexes led to the synthesis of imine (Ampynol (2)) and oxazolidine (Ampyzol (3)) complexes. Besides utilizing of crystallization technique for the separation of the isomers, elemental analysis (CHN), FT-IR, 1H and 13C NMR spectroscopies were used for characterization of the synthesized compounds. In addition, structures of palladium complexes Iminol and Ampyzol were identified by single crystal X-ray diffraction method and investigation of the equilibrium between imine and oxazolidine complexes were carried out by DFT method. The in vitro studies revealed that the compounds have considerable cytotoxicity against human MCF-7 and HeLa cancer cell lines. © 2018 Elsevier Ltd
Publication Date: 2018
Journal of Molecular Structure (00222860)1161pp. 497-511
Eight enantiomerically pure halogenated Schiff base compounds were synthesized by reaction of halogenated salicylaldehydes with 3-Amino-1,2-propanediol (R or S) in water as green solvent at ambient temperature. All compounds were characterized by elemental analyses, NMR (1H and 13C), circular dichroism (CD) and FT-IR spectroscopy. FS-DNA binding studies of these compounds carried out by fluorescence quenching and UV–vis spectroscopy. The obtained results revealed that the ligands bind to DNA as: (R–ClBr) > (R–Cl2) > (R–Br2) > (R–I2) and (S–ClBr) > (S–Cl2) > (S–Br2) > (S–I2), indicating the effect of halogen on binding constant. In addition, DNA-binding constant of the S– and R-enantiomers are different from each other. The ligands can form halogen bonds with DNA that were confirmed by molecular docking. This method was also measured the bond distances and bond angles. The study of obtained data can have concluded that binding affinity of the ligands to DNA depends on strength of halogen bonds. The potential anticancer activity of ligands were also evaluated on MCF-7 and HeLa cancer cell lines by using MTT assay. The results showed that the anticancer activity and FS-DNA interaction is significantly dependent on the stereoisomers of Schiff base compounds as R-enantiomers displayed significantly higher activity than S-enantiomers. The molecular docking was also used to illustrate the specific DNA-binding of synthesized compounds and groove binding mode of DNA interaction was proposed for them. In addition, molecular docking results indicated that there are three types of bonds (H– and X-bond and hX-bond) between synthesized compounds and base pairs of DNA. © 2018 Elsevier B.V.
Publication Date: 2018
Biosensors and Bioelectronics (0956-5663)118pp. 217-223
In the present manuscript, a closed bipolar electrode system integrated with electrochemiluminescence (ECL) detection has been introduced for sensitive diagnosis of human breast cancer cells (MCF-7). For sensitive and selective detection, the anodic pole of the bipolar electrode was modified with the AS1411 aptamer, a specific aptamer for the nucleolin, and treated by the secondary aptamer modified gold nanoparticles. The electrochemiluminescence of luminol was followed in the presence of hydrogen peroxide on the anode pole of bipolar electrode (BPE) as an analytical signal. Moreover, 3D printed microchannels were used for the fabrication of BPE systems to minimize the required amounts of sample. The present aptasensor offers low cost, sensitive and selective cancer cell detection with two acceptable linear ranges. The first linear section appears within 10–100 cells and the latter is found to be within 100–700 cells. The limit of detection was about 10 cells. © 2018 Elsevier B.V.
Publication Date: 2018
ACS Applied Bio Materials (25766422)1(5)pp. 1458-1467
Carbon dots (CDs), as a new generation of fluorescent nanoparticles, have been greatly considered for different biomedical applications. In the present study, a one-pot hydrothermal method was developed for the synthesis of a series of carbon dots (CDs) for cancer imaging and therapy. Taxane diterpenoids were utilized as the carbon source, different diamines were used as the nitrogen source, and folic acid was used as a targeting agent. High-quality photostable and multicolor (blue and green) carbon nanocrystals with a hexagonal shape, a narrow size distribution of less than 20 nm, and high fluorescence quantum yield of up to 50.4% were obtained from taxanes in combination with m-phenylenediamine and folic acid to give the best results. The nanoparticles displayed a potent anticancer activity with IC50 values of 31.3 ± 2.7 and 34.1 ± 1.1 μg mL-1 for the human MCF-7 and HeLa cancer cell lines, respectively, and IC50 value of 120.5 ± 3.8 μg mL-1 on the normal human fibroblast cells. The flow cytometry studies determined apoptosis-mediated cell death as the main anticancer mechanism of CDs, and the molecular studies revealed the induction of both extrinsic and intrinsic apoptosis pathways. The overall results indicated the great potential of synthesized CDs for the simultaneous cancer imaging and therapy. Copyright © 2018 American Chemical Society.
Publication Date: 2018
IET Nanobiotechnology (17518741)12(4)pp. 412-416
High-quality colloidal silver nanoparticles (AgNP) were synthesised via a green approach by using hydroalcoholic extracts of Malva sylvestris. Silver nitrate was used as a substrate ion while the plant extract successfully played the role of reducing and stabilising agents. The synthesised nanoparticles were carefully characterised by using transmission electron microscopy, atomic-force microscopy, energy dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy and UV–vis spectroscopy. The maximum absorption wavelengths of the colloidal solutions synthesised using 70 and 96% ethanol and 100% methanol, as extraction solvents, were 430, 485 and 504 nm, respectively. Interestingly, the size distribution of nanoparticles depended on the used solvent. The best particle size distribution belonged to the nanoparticles synthesised by 70% ethanol extract, which was 20–40 nm. The antibacterial activity of the synthesised nanoparticles was studied on Escherichia coli, Staphylococcus aureus and Streptococcus pyogenes using disk diffusion, minimum inhibitory concentrations and minimum bactericidal concentrations assays. The best antibacterial activity obtained for the AgNPs produced by using 96% ethanolic extract. © The Institution of Engineering and Technology 2017.
Publication Date: 2018
Journal of Molecular Liquids (18733166)264pp. 386-397
As for daily increasing mortality rate in world due to the growth of cancer causing agents, design and synthesis of new compounds with anticancer potential benefits is one of the most important challenges for researchers. In the present work, we synthesized a new Schiff base Pd(II) complex in bulk-scale and also in nano-scales by Sonochemical method. The structure of synthesized complex was determined by single crystal X-ray diffraction technique. Then the cell viability percent of HeLa cancer cells was studied by MTT assay. The results confirmed that reducing the size has salient effect in annihilation of cancer cells. Also, nano-scale complex reached to IC50 in 10 μM of concentration. Binding ability of the nano- and bulk-scale Pd(II) Schiff base complex with calf thymus DNA and human serum albumin was investigated using combination of experimental (fluorescence, circular dichroism (CD) and viscosity) and computational (molecular docking, molecular dynamics simulation and QM/MM) methods. The estimated binding constants for the complex in both of bulk- and nano-scales showed that the nano-scale complex binds more tightly to DNA than its bulk-scale form. This finding is in good agreement with MTT assay results. Molecular docking studies revealed that Pd(II) complex binds to the minor groove and IB binding site of DNA and HSA, respectively. Also, MD simulation studies showed that complexation with the Pd(II) complex changes the structure of HSA with compared to free protein. Finally, the ONIOM results indicated that the structural parameters of the compound changed along with binding to DNA and HSA, indicating the strong interaction between the compound and these biomacromolecules. The values of binding constants depend on the extent of the resultant changes. © 2018
Publication Date: 2017
Journal of Molecular Liquids (01677322)247pp. 238-245
A facile and novel semi-biosynthesis method was reported to develop multifunctional magnetic-plasmonic nanostructures with potent and specific anticancer activity. Silver and gold nanoseeds were synthesized in the presence of Taxus baccata extracted Taxanes, as reducing and capping agents, and used to decorate the silica coated iron oxide nanoparticles. The resulted nanostructures were characterized using UV–Vis spectroscopy, FE-SEM, TEM, FTIR, EDS, and DLS. The resulted hollow and porous nanostructures displayed semi-spherical shapes and sizes between 200 and 500 nm with appropriate magnetic and plasmonic properties. FTIR analysis showed efficient encapsulation of nanostructures by the organic compounds which led to their appropriate colloidal stability. MTT assay and microscopic studies revealed potent and specific anticancer activity of silver decorated magnetic nanostructures (FeAg-Ns), so that after 48 h incubation of human cervical (HeLa) and breast (MCF-7) cancer cells with 100 μg mL− 1 FeAg-Ns up to 70.4 ± 2.02 and 98.6 ± 2.36% mortality were obtained, respectively. Moreover, the nanostructures displayed significantly less cytotoxicity (up to 40.5 ± 1.82%) on noncancerous human fibroblast cells. In conclusion, the synthesized nanostructures have great potential to develop theranostic agents for bio-imaging and magnetically targeted cancer therapy. © 2017
Publication Date: 2017
IET Nanobiotechnology (1751875X)11(4)pp. 370-376
A simple and eco-friendly method for efficient synthesis of stable colloidal silver nanoparticles (AgNPs) using Mentha pulegium extracts is described. A series of reactions was conducted using different types and concentrations of plant extract as well as metal ions to optimize the reaction conditions. AgNPs were characterized by using UV-vis spectroscopy, transmission electron microscopy, atomic force microscopy, dynamic light scattering, zetasizer, energy-dispersive X-ray spectroscopy (EDAX) and Fourier transform infrared spectroscopy (FTIR). At the optimized conditions, plate shaped AgNPs with zeta potential value of-15.7 and plasmon absorption maximum at 450 nm were obtained using high concentration of aqueous extract. Efficient adsorption of organic compounds on the nanoparticles was confirmed by FTIR and EDAX. The biogenic AgNPs displayed promising antibacterial activity on Escherichia coli, Staphylococcus aureus, and Streptococcus pyogenes. The highest antibacterial activity of 25 μg mL-1 was obtained for all the strains using aqueous extract synthesized AgNPs. The aqueous extract synthesised AgNPs also showed considerable antifungal activity against fluconazole resistant Candida albicans. The cytotoxicity assay revealed considerable anticancer activity of AgNPs on HeLa and MCF-7 cancer cells. Overall results indicated high potential of M. pulegium extract to synthesis high quality AgNPs for biomedical applications. © The Institution of Engineering and Technology 2016.
Amiri rudbari, H.,
Mirkhani, V.,
Moghadam, M.,
Tangestaninejad, S.,
Mohammadpoor baltork, I.,
Abbasi kajani, A.,
Azimi gandomani, G. Publication Date: 2017
European Journal of Medicinal Chemistry (02235234)135pp. 230-240
The reaction of a racemic mixture of Schiff base tridentate ligand with vanadium(V) affords homochiral vanadium complex, (VO(R-L))2O and (VO(S-L))2O due to ligand “self-recognition” process. The formation of homochiral vanadium complex was confirmed by 1H NMR, 13C NMR and X-ray diffraction. The HSA- and DNA-binding of the resultant complex is assessed by absorption, fluorescence and circular dichroism (CD) spectroscopy methods. Based on the results, the HSA- and DNA-binding constant, Kb, were found to be 8.0 × 104 and 1.9 × 105 M−1, respectively. Interestingly, in vitro cytotoxicity assay revealed the potent anticancer activity of this complex on two prevalent cancer cell lines of MCF-7 (IC50 value of 14 μM) and HeLa (IC50 value of 36 μM), with considerably low toxicity on normal human fibroblast cells. The maximum cell mortality of 12.3% obtained after 48 h incubation of fibroblast cells with 100 μM of the complex. Additionally, the specific DNA- and HSA-binding was also shown using molecular docking method. The synthesized complex displayed high potential for biomedical applications especially for development of novel and efficient anticancer agents. © 2017 Elsevier Masson SAS
Publication Date: 2017
Langmuir (15205827)33(22)pp. 5565-5576
This study is an attempt to make a step forward to implement the very immature concept of pumpless transportation of liquid into a real miniaturized device or lab-on-chip (LOC) on a plastic substrate. "Inert" plastic materials such as polypropylene (PP) are used in a variety of biomedical applications but their surface engineering is very challenging. Here, it was demonstrated that with a facile innovative wettability patterning route using fluorosilanized UV-independent TiO2 nanoparticle coating it is possible to create wedge-shaped open microfluidic tracks on inert solid surfaces for low-cost biomedical devices (lab-on-plastic). For the future miniaturization and integration of the tracks into a device, a variety of characterization techniques were used to not only systematically study the surface patterning chemistry and topography but also to have a clear knowledge of its biological interactions and performance. The effect of such surface architecture on the biological performance was studied in terms of static/dynamic protein (bovine serum albumin) adsorption, bacterial (Staphylococcus aureus and Staphylococcus epidermidis) adhesion, cell viability (using HeLa and MCF-7 cancer cell lines as well as noncancerous human fibroblast cells), and cell patterning (Murine embryonic fibroblasts). Strategies are discussed for incorporating such a confined track into a diagnostic device in which its sensing portion is based on protein, microorganism, or cells. Finally, for the proof-of-principle of biosensing application, the well-known high-affinity molecular couple of BSA-antiBSA as a biological model was employed. © 2017 American Chemical Society.
Publication Date: 2017
Journal of Molecular Liquids (18733166)248pp. 24-35
Herein, we synthesized a new nano-scale Schiff base Ni(II) complex in water as a green solvent and at ambient temperature. The compound was characterized using FT-IR and elemental analysis. Also, its molecular structure was determined by single crystal X-ray diffraction technique. The MTT assay results indicated that the anticancer activity of the compound is affected by its size. Finally, binding ability of the nano-scale Ni(II) Schiff base complex with calf thymus DNA and human serum albumin was investigated using combination of experimental (UV–Vis, fluorescence, circular dichroism (CD) and viscosity) and computational (molecular docking, molecular dynamics simulation and qm/mm) methods. The estimated binding constants for the DNA-complex and HSA-complex were about 104 M− 1. Molecular docking studies revealed the binding of Ni(II) complex to the minor groove of DNA and warfarin binding site of protein by formation of hydrogen bond, π-cation and hydrophobic interactions. MD simulation studies revealed that complexation with NiL2 changed the structure of HSA when compared to free protein. Finally, the ONIOM results showed that the structural parameters of the compound changed along with binding to DNA and HSA, indicating the strong interaction between the compound and these biomacromolecules. © 2017 Elsevier B.V.
Publication Date: 2016
Journal of Molecular Liquids (01677322)223pp. 549-556
Cancer is one of the main causes of human fatality and finding effective anticancer drugs is a high priority. Modified anticancer medicines have advantages compared to traditional drugs including; better delivery and lower doses. In the present study, the anticancer activity of silver nanoparticles (Ag NPs) synthesized using Taxus baccata extracts was studied on Caov-4 and HeLa cancer cell lines as well as normal human fibroblast cells. Microscopic studies showed significant morphological changes of cancer cells following the exposure to the Ag NPs while MTT assay revealed dose, time and cell line dependent cytotoxicity. The toxic effect of Ag NPs on Caov-4 cells was considerably higher than HeLa cells as > 98% mortality obtained after 72 h incubation of Caov-4 cells with 5 and 20 μg/mL Ag NPs encapsulated by aqueous and ethanolic extract, respectively. Interestingly, aqueous extract encapsulated Ag NPs had no significant toxicity on normal fibroblast cells suggesting potent and selective anticancer activity on cancer cell lines. Flow cytometric analysis revealed a combination of apoptosis and necrosis following the exposure of Ag NPs to cancer cells. Apoptosis was determined as main mechanism of cell death in low concentration of Ag NPs while considerable necrosis (up to 41%) was observed by increasing the Ag NPs dose (up to 10 μg/mL) and incubation time (up to 72 h). The significant DNA damage and change in expression level of caspase 8, caspase 9, bcl-2 and c-Abl genes indicated the induction of mitochondrial death pathway following cell exposure to Ag NPs. It can be concluded that the preparation method and stabilizing agents may have major effects on the biological activity of nanoparticles as well as their physicochemical properties. © 2016 Elsevier B.V.
Publication Date: 2016
Journal Of The Iranian Chemical Society (1735207X)13(10)pp. 1895-1904
Polyoxometalates (POMs) are negatively charged clusters consisting of transition metals and oxygen atoms. The antiviral and antitumor activities are the dominant activities of POMs in pharmacology and medicine. Based on Co-containing Keggin polyoxomolybdate (K6[SiMo11O39Co(H2O)].nH2O), nanosized starch, and lipid-encapsulated derivatives (abbreviated as SiMo11Co, SEP and LEP, respectively) were synthesized and characterized by FT-IR spectroscopy, ICP, TG analysis, SEM and TEM images. The results show that the SiMo11Co retains its parent structure after encapsulation by starch and lipid nanoparticles. The biological activity of SiMo11Co has been evaluated by investigating its binding ability to calf thymus DNA (ctDNA), using UV–Vis absorption spectroscopy, fluorescence quenching and fluorescence Scatchard plots. The obtained results of absorption titration rule out the intercalating binding mode and propose the groove or outside stacking binding for SiMo11Co. These results were authenticated by fluorescence quenching experiments and scatchard plots. Absorption spectral traces reveal 10.21 % hyperchromism for SiMo11Co. The value of 7.6 × 103 M−1 was obtained for binding constant (Kb) of SiMo11Co to ctDNA. Furthermore, the in vitro antitumor activity of SiMo11Co and nano-encapsulated forms was investigated using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay that was carried out on two types of human cancer cells, MCF-7 (breast cancer cells) and HEK-293 (Human Embryonic Kidney). The results represent the enhancement of cell penetration and antitumor activity of SiMo11Co due to its encapsulation in starch or lipid nanoparticles. However, this observed enhancement for the lipid relative to the starch nanocapsule can be attributed to its smaller size. © 2016, Iranian Chemical Society.
Publication Date: 2016
RSC Advances (20462069)6(68)pp. 63973-63983
Biogenic high quality colloidal gold nanoparticles (AuNPs), as one of the best anticancer theranostic nanostructures among those reported to date, were synthesized via a simple, efficient and green chemical method using aqueous and ethanolic Taxus baccata extracts and comprehensively characterized by UV-vis spectroscopy, TEM, SEM, AFM, DLS, Zetasizer, EDS, and FT-IR techniques. The AuNPs, encapsulated by the ethanolic compounds of T. baccata with different shapes and uniform size of less than 20 nm and polydispersity index of 0.276, were found completely stable for several months. In consistent with the results of microscopic observations of cell morphology, MTT assay revealed a potent, selective, dose- and time-dependent anticancer activity of AuNPs on the prevalent cancer cell lines including breast (MCF-7), cervical (HeLa) and ovarian (Caov-4), more effective than most of the other reported similar cases. The detailed in vitro investigation of cell exposure by AuNPs, using flow cytometry and real-time PCR, indicated caspase-independent death program as most probable anticancer mechanism of AuNPs. The overall results firmly indicated that the organic compounds of T. baccata, as appropriate reducing and stabilizing agents, not only significantly affect the physicochemical properties of AuNPs but also led to the green synthesis of potent anticancer agent with high potential for cancer therapy. © 2016 The Royal Society of Chemistry.
Publication Date: 2016
Journal of Experimental Nanoscience (17458080)11(14)pp. 1087-1109
Advanced surface modification approaches of biomaterials alongside the advent of sophisticated analytical techniques have provided a great opportunity to understand how the physicochemical characteristics of materials determine cell–surface dynamics at molecular and atomic scale. However, there are still many contradictory reports, which are mainly due to inadequate information about the role of the two parameters of surface chemistry and structure and their synergistic effect as an adequate predictor of biological performance. Here, surface parameters were altered by grafting of poly ethylene glycol (PEG) on polyurethane (PU) surfaces through a superhydrophilic modification method. In this study, surface modification of PU films by PEG thin layer via grafting technique and TiO2 nanoparticle entrapment in the brush polymers was investigated. The surface modification led to a reduction in protein adsorption and bacterial attachment by 8.7 times and 71% respectively with no cytotoxicity effect on HeLa cells. It was also observed that when PU surface became superhydrophilic the bacterial adhesion becomes independent of bacterium type. In general, it was observed that the impact of topographical changes on the biocompatibility and biofilm formation becomes significantly more profound than that of the surface chemistry alteration. © 2016 Informa UK Limited, trading as Taylor & Francis Group.
Amiri rudbari, H.,
Mirkhani, V.,
Moghadam, M.,
Tangestaninejad, S.,
Mohammadpoor baltork, I.,
Azimi gandomani, G.,
Abbasi kajani, A. Publication Date: 2016
Journal of Photochemistry and Photobiology B: Biology (18732682)163pp. 246-260
A racemic mixture of a new chiral Schiff base ligand (HL: R/S-(1-phenylethylimino)methylnaphtalen-2-ol) has been utilized to prepare Pd(II) complex. Crystallization technique has been employed to separate diastereomeric pairs of Pd(II) complex: (meso PdL2) and (rac PdL2) that in this paper are known as PdL2 1 and PdL2 2, respectively. The synthesized complexes have been characterized by means of elemental analysis (CHN), FT-IR, 1H and 13C NMR spectroscopies. Moreover, PdL2 1 has been structurally characterized by single-crystal X-ray diffraction. The geometry around the metal center is square-planar. The interaction of two diastereomers of Pd(II) complex with FS-DNA has been explored, using UV–vis spectroscopy, fluorescence quenching, chemometrics and viscosity measurement methods. The PdL2 1 exhibited higher binding constant, about 10-fold, (1.0 × 106 M− 1) as compared to PdL2 2 (1.5 1.5 × 105 M− 1). Moreover, the human serum albumin (HSA) binding ability has been monitored by absorption, quenching of tryptophan fluorescence emission and circular dichroism (CD) studies. The slight difference is observed between HSA binding affinity with the complexes: PdL2 1 (6.2 × 104 M− 1) and PdL2 2 (3.3 × 104 M− 1). Also, the thermodynamic parameters were determined at three different temperatures (298, 308 and 318 K). In this study, molecular docking was also carried out to confirm and illustrate the specific DNA- and HSA-binding of the Pd(II) complexes. In the PdL2 1-HSA system a T-shaped π-π interaction with PHE206 was observed. While in the PdL2 2-HSA system there are a hydrogen bond, a π-cation and two T-shaped π-π interactions with ASB324, LYS212 and PHE228, respectively. The groove binding mode of DNA interaction has been proposed for both diastereomers. © 2016 Elsevier B.V.
Publication Date: 2014
RSC Advances (20462069)4(106)pp. 61394-61403
Anisotropic silver nanoparticles with controlled shape and size were synthesized by a simple, efficient and eco-friendly method using Taxus baccata extracts as reducing, capping and stabilizing agents. The monodispersed nanoparticles showed narrow size distribution with an average of 75.1 nm and high stability in aqueous colloidal solutions. Different factors including the concentration and the type of plant extract, the concentration of silver nitrate, pH and temperature were investigated in order to synthesize silver nanoparticles of desired size and shape. Surprisingly, the type of plant extract was the main parameter and remarkably affected the physical, chemical and cytotoxic properties of the nanoparticles. On the basis of AFM and TEM results, the use of an aqueous extract led to the synthesis of spherical nanoparticles while stable colloidal silver nanoparticles of hexagonal and truncated triangular shapes with characteristic absorption maxima of 530 and 690 nm, respectively, were synthesized in the presence of high concentrations of ethanolic extract. The strong adsorption of Taxus metabolites on the surfaces of nanoparticles was confirmed by FTIR and TGA analyses. The MTT assay revealed potent anticancer effects of the aqueous extract synthesized nanoparticles on MCF-7 cells with an IC50 value of 0.25 μg mL-1 after 48 hours incubation time. According to the results, the present reported biogenic method has great potential for simple and efficient development of novel multifunctional nanoparticles for tracking, imaging, and therapy of cancer cells. This journal is © The Royal Society of Chemistry 2014.
Publication Date: 2013
Iranian Journal Of Biotechnology (17283043)11(2)pp. 96-103
Background: Taxol is one of the most important anti-cancer drugs, which is obtained from yew trees (Taxus sp.). T+he first step in side chain assembly of taxol is catalyzed by phenylalanine aminomutase, which converts α-phenylalanine to β-phenylalanine. Objectives: In this study, for the first time, we report on the cloning, preliminary expression and characterization of a full-length gene and cDNA encoding phenylalanine aminomutase from Taxus baccata L. Materials and Methods: Comparison of the full-length gene with other ones identified from the Taxus species showed high similarity, particularly with Taxus x media. Results: The results showed that the expression level of this gene in Taxus baccata is very low and therefore this enzymatic step could be a rate limiting step in the taxol biosynthesis pathway. Successful amplification of the cDNA was only obtained from RNA samples isolated from methyl jasmonate elicited suspension cells of Taxus baccata. The cloned cDNA contained a 2064 bp open reading frame encoding a protein composed of 687 amino acids. Sequence comparison analysis revealed that the gene is very similar (98 - 99 %) with respect to the nucleotide and amino acid sequences in different Taxus species and also share the signature active site motif (175ASG177). Conclusions: The predicted structure of TbPAM was analyzed using bioinformatic tools. The results indicated that the protein has similar overall folding to tyrosine aminomutase. © 2013, National Institute of Genetic Engineering and Biotechnology; Published by Kowsar Corp.
Publication Date: 2012
Biotechnology and Applied Biochemistry (14708744)59(3)pp. 223-227
The ability of an aprotic solvent, dimethylsulfoxide (DMSO), to induce taxane synthesis and release from cell suspension culture of Taxus baccata was examined. The results showed that applying DMSO in optimal conditions not only led to enhancement in taxane excretion from the cells but also led to improvement in taxol synthesis. Maximum yields for taxol [3.34mg/g dry cell weight (DCW)] were achieved by adding 5% DMSO to the culture at the late-exponential phase of cell growth (day 14) and culturing for 21 days, which was 2.26-fold of that for the control (1.48mg/g DCW). However, adding 5% DMSO did not affect the yield of 10-deacetyl baccatin III and baccatin III. This condition also resulted in maximum extracellular taxane (4.86mg/L); this value was 2.82-fold higher than that in the control (1.72mg/L). We demonstrated that the late-exponential phase of cell growth could be the best time to add elicitor for maximizing the yield of taxol. Adding DMSO at earlier times (days 1 and 7) or in other concentrations (1% and 3%) had negative effects on taxane synthesis. Overall results suggest that DMSO has good potential to enhance synthesis and release taxol from cell suspension culture of T. baccata L. © 2012 International Union of Biochemistry and Molecular Biology, Inc.
Publication Date: 2012
DARU, Journal of Pharmaceutical Sciences (20082231)20(1)
Background and purpose of the study. Taxol is one of the most effective anticancer drugs that isolated from Taxus sp. due to the slow growth of Taxus trees and low concentration of Taxol in the tissues, the biotechnological approaches especially plant cell culture have been considered to produce Taxol in commercial scale. Methods. We investigated the effects of basal medium type used in culture media on production of Taxol and other taxane compounds from cell suspension culture of T. baccata L. Briefly, five commonly basal media including Gamborg, Murashige and Skoog, Woody Plant, Schenk and Hildebrandt, and Driver and Kuniyuki medium were used for preparing separate suspension culture media. The intra- and extra-cellular yields of taxanes were analyzed by using HPLC after 21 days period of culturing. Results: The yields of taxanes were significantly different for the cultures prepared by different basal media. Moreover, the effects of basal medium on the yield of products differed for varius taxane compounds. Maximum yields of Baccatin III (10.03 mgl-1) and 10-deacetyl baccatin III (4.2 mgl-1) were achieved from the DKW basal media, but the yield of Taxol was maximum (16.58 mgl-1) in the WPM basal media. Furthermore, the secretion of taxanes from the cells into medium was also considerably affected by the type of basal medium. The maximum extra-cellular yield of Taxol (7.81 mgl-1), Baccatin III (5.0 mgl-1), and 10-deacetyl baccatin III (1.45 mgl-1) were also obtained by using DKW basal medium that were significantly higher than those obtained from other culture media. © 2012 Kajani et al.; licensee BioMed Central Ltd.
Publication Date: 2010
Molecular Biology Reports (15734978)37(2)pp. 797-800
Improved and efficient methods were developed for isolating high quality DNA and RNA from different sources of Iranian Yew (Taxus baccata L.). The methods were based on CTAB extraction buffer added with high levels of polyvinylpyrrolidone (PVP) and β-mercaptoethanol to properly remove polysaccharides and prevent oxidation of phenolics. The pellets obtained by ethanol precipitation were washed only with Chloroform: isoamyl alcohol (24:1). So, we could successfully eliminate the dangerous phenol/chloroform extraction steps from the isolation procedure. Both spectrophotometric (A 260/A280 and A260/A230 ratios) and agarose electrophoresis analysis of isolated nucleic acids (DNA and RNA) indicated good results. DNA with the average yield of 100-300 μg/g leaf and stem tissue and total RNA with an average yield of 20-30 μg/g cell culture and 80-100 μg/g leaf and stem tissue of Iranian yew could be obtained. Successful amplification of pam and pds by PCR and RT-PCR, showed the integrity of isolated DNA and RNA, respectively. © Springer Science+Business Media B.V. 2009.
Publication Date: 2010
Biotechnology and Applied Biochemistry (08854513)56(2)pp. 71-76
The ability of a potent hydrophobic adsorbent, AC (activated charcoal), to induce total taxane synthesis and to improve the recovery of the products from suspension culture of Taxus baccata L. was examined. The results strongly showed that the application of encapsulated AC as an adsorbent not only led to improvement of in situ recover but also led to enhancement in taxane synthesis within the cells aswell as having no inhibitory effects on the cell growth. The maximum yields for 10-deacetyl baccatin III, baccatin III and taxol were achieved by adding 1 g·l-1 adsorbent as alginate beads to the culture and culturing for 21 days and these were 3.5-, 4- and 2-fold respectively of that for the control.We demonstrated that the late exponential phase of the cell growth (day 14) could be the best time to add the adsorbent for maximizing the yield of taxol. Adding 1 g·l-1 AC resulted in maximum extracellular taxol (5.584 mg·l-1); this value was 2.19-fold higher than that in the control. According to the present results, AC is a powerful adsorbent that efficiently adsorbs the taxane compounds from a medium. We suggested that encapsulated AC could be used as a promising adsorbent agent for large-scale taxane production when it is added in combination with proper elicitors. © 2010 Portland Press Limited.
